Fraser: Cultures of Heteroecious Rusts 229 



Plowright in 1890, using telial material of Uromyces Scirpi 

 Burr, from Scirpus maritimus L. in England, successfully infected 

 Glaux maritima. This work has not been confirmed in America, 

 but American aecia in Glaux have been referred to that species. 

 This experiment and the one described below shows that aecia on 

 Glaux may belong to P. Distichlidis or P. subnitens. 



Uromyces Alopecuri Seym. 



Inoculations were made on a pot of Alopecurus aristulatus 

 Michx. with aeciospores from aecia on Ranunculus apetalus Farr, 

 collected by Mr. A. McNeil. Uredinia and telia followed. This 

 confirms the results of cultures in 1918. (See Mycol. 11 : 129. 

 1919.) 



PUCCINIA SUBNITENS Diet. 



Field observations near Saskatoon in 1920 indicated that aecia 

 .on Glaux maritima were connected with Puccinia subnitens or Dis- 

 tichlis stricta (Torr.) Rydb. Inoculations with germinating telio- 

 spores were made on two pots of Glaux maritima on June 26. 

 Pycnia appeared in about a week, and were followed by abundant 

 aecia. The experiments were repeated in 192 1. Inoculations 

 were made on different potted plants on May 27, June 2 and 9. 

 Heavy infection followed in all the plants inoculated, both pycnia 

 and aecia developing abundantly. Inoculations on Dodccatheon 

 pauciflorum (Durand) Greene failed to produce infection. 



Aecia from the cultures and field collections of 1920 were sent 

 to Dr. Arthur, who was then {preparing the manuscript of North 

 American Flora covering the heteroecious grass rusts. He studied 

 the field collections and the cultures of aecia and reported that they 

 agreed in every respect with those of P. subnitens, and pointed out 

 that the outer walls of the peridial cells were much thickened as in 

 the typical aecia of P. subnitens. In this respect they differed 

 markedly from the aecia on Glaux belonging to P. Distichlidis. 

 He listed the field collections on Glaux from Saskatchewan under 

 P. subnitens Diet. (Dicaeoma Sarcobati (Peck) Arth.). (See 

 N. A. F. 7: Part 4, 3<>5-) 



The aecia of P. subnitens on Glaux were long and slender and 

 differed in this respect from the aecia of P. Distichlidis on the 



