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Mycologia 



spores were either placed or allowed to fall on the moistened sur- 

 faces, especially the under one, of the leaves ; the plants were then 

 kept in moist chambers or iceless refrigerators 7 2 to 4 days, then 

 placed in rooms or compartments of the greenhouse separate from 

 plants inoculated with other species of Coleosporium. In inocu- 

 lations with sporidia, whole infected plants or the leaves of in- 

 fected plants were either suspended over the pine trees or laid on 

 a wire netting over them in a large moist chamber or an iceless 

 refrigerator for 2 to 4 days. The iceless refrigerator is the best 

 form of inoculating chamber that we have used, more especially in 

 warm weather. An equal number of control plants were placed 

 uninoculated under similar conditions, apart from the inoculated 

 sets of plants. Plants grown from healthy cuttings or from seed 

 in the greenhouses were used. In each experiment given in this 

 series of papers the control plants remained healthy. 



In the aecial inoculations with Coleosporium helianthi, aecio- 

 spores from aecia collected 8 on Pinus virginiana from the follow- 

 ing localities were used separately in the experiments : Greenwood 

 Furnace, Pa. ; Washington, D. C. ; Chain Bridge, Va. ; Black 

 Mountain and Marion, N. C. ; Greenville, S. C. ; and Rome, Ga. 

 Plants as follows were inoculated: 1 Aster cordifolius* 1 A. 

 laevis, 1 A. undulatus, 9 Coreopsis major, 10 C. verticillata, 1 C. 

 tripteris, 1 Chrysopsis mariana, 16 Helianthus decapetalus, 6 H. 

 divaricatus, 2 H. dowellianus, r H. giganteus, 2 H. glaucus, 16 

 H. hirsutus, 3 H. microcephalics, 2 H. radula, 1 Laciniaria elegans, 

 3 Parthenium integri folium, 1 Rudbeckia laciniata, 2 Silphium 

 asteriscus, 2 S. integri folium, 1 5. perfoliatum, 1 S, trifoliatum, 



7 Hunt, N. Rex. The " Iceless Refrigerator " as an Inoculation Chamber. 

 Phytopathology 9: 211-212. May, 1919. 



8 Unless otherwise credited all collections used in inoculations and noted in 

 this series of papers were made by the senior writer. 



9 Unless the authority is designated, the names used for species of plants 

 from the southeastern United States are those used by Small, J. H., Flora of 

 the Southeastern United States, 1913. For those from the northeastern United 

 States, Britton, N. L., & Brown, A., Illustrated Flora of the Northeastern 

 United States, etc., 1898. For those from the Rocky Mountain region, Ryd- 

 berg, P. A., Flora of Colorado. For those from the northwestern United 

 States, Piper, C. V., Flora of Washington, Contributions from the National 

 Herbarium, Vol. XI, 1906. 



