Hedgcock and Hunt: Notes on Coleosporium 247 



i Solidago canadensis, 2 Verbesina virginica and 1 Vernonia nove- 

 boracensis. Of these plants, only those of species of Helianthus 

 became infected, bearing mature uredinia in 12 to 15 days and 

 mature telia in 6 weeks to 2 months. The number of plants in- 

 fected of each species was as follows : 5 H. decapetalus, 3 H. 

 divaricatus, 1 H. giganteus, 2 H. glaucus, 13 H. hirsutus, 1 H. 

 microcephalus and 2 H. radula. On the last-named species the 

 rust is now reported for the first time. Of the 20 plants of species 

 of Coreopsis inoculated, none were infected. 



Inoculations were made with the urediniospores of Coleosporium 

 helianthi from Helianthus hirsutus obtained from previous inocu- 

 lations June 10, 1919, on the following plants : 2 H. hirsutus, 2 

 H. radula, 2 Coreopsis major and 4 C. verticillata. All the plants 

 of Helianthus became infected, bearing mature uredinia June 26 

 and telia July 20. All plants of Coreopsis remained free from 

 infection. 



Pine trees were inoculated September 29, 1920, with the sporidia 

 from the telia of Coleosporium helianthi on Helianthus decapetalus 

 collected the previous day near Chain Bridge, Va. The following 

 trees were inoculated : 1 P. caribaea, 1 P., edulis Engelm., 1 P. 

 glabra, 2 P. radiata Don. and 8 P. virginiana. Seven trees of the 

 last-named species were infected, many mature pycnia appearing 

 on the needles by March 21, 1921, and abundant mature aecia by 

 April 20. The other trees remained uninfected. 



Aeciospores of Coleosporium helianthi from the preceding ex- 

 periment were inoculated April 20, 1921, on the following plants: 



1 Coreopsis major, 2 C. verticillata, 1 Helianthus divaricatus and 



2 H. hirsutus. Only the three plants of Helianthus were infected 

 with the Coleosporium, producing both the uredinial and telial 

 stages. 



Inoculations with Coleosporium inconspicuum 



In the aecial inoculations with Coleosporium inconspicuum, 

 aeciospores from aecia collected on Pinus virginiana from the fol- 

 lowing localities were used separately in the experiments : Takoma 

 Park, Md. ; Washington, D. C. ; Roanoke, Va. ; Asheville, Black 

 Mountain and Hot Springs, N. C. Plants as follows were inocu- 



