248 



Mycologia 



lated : i Chrysopsis mariana, I Coreopsis lanceolata, 16 C. major, 

 i C. tripteris, 26 C. verticillata, 1 Elephant opus carolinianus, 10 

 Helianthus decapetalus, 1 H. divaricatus, 2 H. dowellianus, 1 H. 

 glaucus, 10 H. hirsutus, 4 H. microcephalus, 1 H. occidentalis, 1 

 H. tuberosus, 1 Laciniaria elegans, 2 Silphium integrifolium, 4 

 Verbesina virginica, 2 Vernonia blodgettii, 3 flaccidi folia, 1 

 F. glauca, 2 F. oligantha and 2 F. noveboracensis. Of these 

 plants, only those of species of Coreopsis became infected, many 

 of them abundantly, bearing mature uredinia in 13 to 16 days, and 

 mature telia in about 2 months. The number of plants of each 

 species infected was as follows : 1 C. lanceolata, 7 C. major, 20 



C. verticillata and 1 C. tripteris. 



Aeciospores of Coleosporium inconspicuum from aecia on Pinus 

 echinata collected near Mt. Airy, N. C, were used March 22, 1919, 

 to inoculate plants as follows : 2 Coreopsis major, 1 C. verticillata 

 and 1 Helianthus hirsutus, and on the same date a duplicate set of 

 plants was inoculated with aeciospores from a collection of aecia 

 on Pinus palustris made near Styx, S. C, May 11. In each of 

 these two experiments, only the plants of C. verticillata became 

 infected with the Coleosporium, proving it to be C. inconspicuum 

 in each case. 



Pine trees were inoculated September 29, 1920, with the sporidia 

 from the telia of Coleosporium inconspicuum on Coreopsis verticil- 

 lata, collected the preceding day in Virginia, near Washington, 



D. C. The following trees were inoculated : 1 Pinus glabra, 4 

 P. virginiana. All the trees of the latter species were infected, 

 bearing mature pycnia on the needles by March 2, 1921, and 

 mature aecia by April 20. 



Aeciospores of Coleosporium inconspicuum from the preceding 

 experiment were inoculated April 20, 1921, on the following 

 plants : 1 Coreopsis major, 6 C. verticillata and 2 Helianthus hir- 

 sutus. The one plant of C. major and 4 of C. verticillata were 

 infected with the Coleosporium, producing both the uredinial and 

 telial stages. 



All our inoculations fail to furnish the slightest proof that 

 Coleosporium helianthi and C. inconspicuum are identical physio- 

 logically, but on the contrary indicate that they are distinct species. 



