252 



Mycologia 



In the study of Coleosporium terebinthinaceae the following 

 inoculations have been made : 



During May, 1916, and April, 1918, 7 sets of separate inocula- 

 tions were made with aeciospores from collections made on Pinus 

 echinata at Auburn, Ala. ; Gainesville, Ga. ; Clearwater, S. C. ; and 

 Marion, N. C. Plants of the following species were inoculated: 

 3 Amsonva ciliata, 2 Coreopsis verticillata, 2 Laciniaria gr ami- 

 folia, 10 Parthenium integri folium, 1 Silphium asteriscus, 13 S. 

 integrifolium and 3 5*. trifoliatum. The following plants were 

 infected, some of them heavily, bearing mature uredinia in 11 to 

 13 days and telia in about 2 months: 1 Silphium asteriscus, 4 S\ 

 integrifolium, 3 S. trifoliatum and 1 Parthenium integrifolium. 



Urediniospores obtained from Silphium integrifolium in one of 

 the preceding inoculations were used June 27, 191 6, to inoculate 

 plants as follows : 2 Silphium integrifolium, 1 vS\ trifoliatum and 

 2 Parthenium integrifolium. One plant each of S. integrifolium 

 and S\ trifoliatum were infected heavily, bearing mature uredinia 

 in 13 days and mature telia in about 2 months. 



A number of inoculations were made with telia on several species 

 of pine during 191 5 and 1916 without infection. 



Although plants of but few species have been tested by inocula- 

 tion with the aeciospores of C. terebinthinaceae, a large number of 

 plants of species of Silphium and Parthenium susceptible to this 

 species of Coleosporium have been tested, without infection, by 

 inoculation with the aeciospores of the following species of Coleo- 

 sporium: C. carneum (Bosc.) Jackson, C. elephant opodis (Schw.) 

 Thiim., C. helianthi (Schw.) Arthur, C. inconspicuum (Long) 

 Hedge. & Long, C. ipomoeae (Schw.) Burrill, C. minutum Hedge. 

 & Hunt, and C. solidaginis (Schw.) Thiim. 



Coleosporium terebinthinaceae according to our records has been 

 collected in the United States as follows : 



O and I on Pinus: 



P. echinata: Alabama, Georgia, North Carolina and South Carolina. 



P. palustris : South Carolina. 



P. rigida : North Carolina. 



P. serotina: South Carolina. 



P. taeda: Alabama and South Carolina. 



II and III on Parthenium and Silphium: 



P. integrifolium: Alabama, Georgia, North Carolina, Tennessee and Virginia. 



