Hedgcock and Hunt: Notes on Coleosporium 255 



April 3, 1914, aeciospores from aecia collected on Pinus serotina 

 by Dr. Long at St. Augustine, Fla., March 30, were used to inocu- 

 late plants of the following species : 2 Euthamia graminifolia and 



2 Solidago rugosa., Only the plants of Euthamia were infected 

 and bore uredinia and telia as before. 



During March and April, 1914, aeciospores from aecia collected 

 on Pinus toe da by Dr. Long at Brooksville and St. Augustine, Fla, r 

 and Henry, S. C, were used to inoculate plants of the following 

 species : 9 Euthamia graminifolia, 1 Helianthus annuus, 1 Solidago 

 rugosa and 3 S. speciosa. Only the plants of Euthamia became 

 infected and bore uredinia and telia as before. 



During April, 191 5, aeciospores from aecia collected on Pinus 

 echinata at Florence, S. C, were used to inoculate and infect 1 

 plant of Euthamia caroliniana and 2 of E. graminifolia, which as 

 a result bore uredinia and telia. Also aeciospores from aecia col- 

 lected on Pinus caribaea at Jacksonville, Fla., were used to infect 



3 plants of Euthamia graminifolia which bore uredinia and telia 

 as before. 



During June, 1916, aeciospores from aecia on Pinus resinosa 

 collected by Dr. P. Spaulding at Sharon, Vt., were used to 

 infect Euthamia graminifolia, which as a result bore uredinia and 

 telia. Plants of Euthamia infected during 1914, 191 5, and igi6 y 

 bore mature uredinia in 11 to 18 days and mature telia in 5 to 

 8 weeks. 



Inoculations with the sporidia from the telia of Coleosporium 

 delicatulum have been made on pine trees as follows : 



September 11, 1916, the following were inoculated with sporidia 

 from telia collected September 10 near Takoma Park, D. C. : 1 

 Pinus caribaea, 18 2 P. clausa, 1 P. contorta, 1 P. coultcri, 1 P. dcnsi- 

 flora Thunb., 2 P. echinata, 2 P. edulis, 3 P. glabra, 2 P. mayriana 

 Sudw., 1 P. monophylla, 1 P. montana Mill., 1 P. palustris, 2 P. 

 rigida, 1 P. scopulorum, 2 P. serotina, 2 P. taeda and 1 P. thun- 

 bergii Pari. Of these trees the following were infected, bearing 

 mature pycnia in January and mature aecia in March, 1917: 1 P. 

 contor*ta, 2 P. echinata, 2 P. glabra, 1 P. mayriana, 1 P. palustris, 

 2 P. rigida, 2 P. serotina and 1 P. taeda, 



is In these two papers, P. heterophylla is considered synonymous with 

 P. caribaea, and P. murrayana with P. contorta. 



