Hedgcock & Hunt: Coleosporium 305 



were infected as follows: 3 vS\ bicolor, 2 5\ canadensis, 3 5". 

 multiradiata, 1 S. riddellii, and 1 vS\ squarrosa. Uredinia and 

 telia were produced in the usual time. 



Four sets of inoculations were made with aeciospores from 

 aecia collected on the needles of Pinus resinosa from Itasca Park, 

 Minn, (collected by Mr. R. G. Pierce) ; Sharon, Vt. (collected by 

 Dr. P. Spaulding) ; and Caledonia, Pa. Plants as follows were 

 inoculated: 1 Aster conspicuus, 1 A. cordifolius, 1 A. macrophyl- 

 lus, 1 A. undulatus, 3 Campanula rapunculoides, 1 Convolvulus 

 sepium, 1 Coreopsis verticillata, 1 Helianthus decapetalus, 1 

 Senecio aureus, 1 S. obovatus, 2 Solidago canadensis, 6 5. mul- 

 tiradiata, 3 S. riddellii, and 1 S. squarrosa. Of these plants, 

 only those of species of Solidago as follows were infected : 2 

 S. canadensis, 5 5. multiradiata, 1 vS\ riddelli, and 1 vS\ squarrosa. 

 Uredinia and telia were produced in the usual time. 



Two sets of inoculations were made February 26 and March 

 15, 1 921, with aeciospores from aecia on Pinus radiata infected 

 artificially October 7, 1920, with sporidia from teliospores from 

 Solidago bicolor. The following plants were inoculated : 3 Aster 

 laevis, 6 Chrysopsis mariana, 2 Solidago bicolor, 1 5*. monticola, 

 and 6 vS\ multiradiata. Only the plants of Solidago bicolor and 

 S. multiradiata were infected, bearing mature uredinia in about 

 20 days, and mature telia in about 3 months. These inoculations 

 were made at lower temperatures than those with aeciospores 

 from species of pine, which were made chiefly in May and June. 

 This explains the longer time required for the production of 

 mature uredinia and telia. 



One set of inoculations was made July 7, 1914, 18 with aecio- 

 spores from aecia collected by H. E. West of the Forest Service, 

 on Pinus contort a, near Bozeman, Mont., June 25. The follow- 

 ing plants were inoculated: 2 Aster conspicuus, 1 A. cordifolius, 

 2 A. geyeri, 2 Coreopsis verticillata, 2 Elephantopus tomentosus, 

 2 Helianthus divaricatus, 2 Solidago canadensis, 2 S. juncea, 2 

 S. multiradiata, and 2 Vernonia glauca. Of these plants, only 

 those of Aster conspicuus and A. cordifolius were infected. 



During 191 4 to 1920, fifteen sets o f inoculations were made, 

 using urediniO'Spores grown in pedigreed cultures from aecio- 



18 Hedgcock, G. G. L. c. 



