298 



Mycologia 



Another collection sent from Valentine, Neb., by Rev. J. M. 

 Bates, was sown May 26, on R. Cynosbati, B. aster oides, U. 

 gracilis and E. argentea. All the sowings were without effect. 

 All three collections gave fairly strong germination of the telio- 

 spores at the date of sowing. 



Cultures have been attempted once before 4 on five hosts, all 

 but one different from the above. The morphological characters 

 of this form approximate those of Puccinia Grossulariae on 

 various species of Carex, but nothing has yet been proven by 

 cultures. 



Successful cultures in 1916 supplementing previous 

 work. — The culture of the following seven species adds valu- 

 able information in each case to the facts previously recorded. 



1. Puccinia universalis Arth. — Material on Carex filifolia, 

 collected by the writer May 18, 191 6, at Ogallala, Neb., was sown 

 June 2, on Artemisia gnaphalodes. Pycnia were detected June 

 15, but doubtless appeared earlier, being obscured by the heavy 

 tomentum of the plant, and were followed by a large number of 

 aecia, first seen June 28. This rust has been cultivated a number 

 of times before 5 on various species of Artemisia, but only with 

 telial material from Carex stenophylla. Carex filifolia, like C. 

 stenophylla, occurs on semi-arid plains. It usually grows only 

 three or four inches high, and in dense mats. It is locally known 

 as " nigger-wool " or " black-root," from the tough, tangled mass 

 of fine, black roots, resisting decay for a year or two when turned 

 up by the plow and exposed to weathering. 



2. Puccinia poculiformis (Jacq.) Wettst. — This very com- 

 mon stem rust of grasses and grains was grown on Berberis vul- 

 garis from two collections made by Mr. G. E. Osterhout, 

 Windsor, Colo., on March 2, one from Sporobolus cryptandrus, 

 sown April 23, showing pycnia May 6, but not developing aecia, 

 and the other from Elymus canadensis, sown May 15, showing 

 pycnia May 25 and aecia June 6. Another collection on Agro- 

 pyron Smithii made by Mr. E. Bethel, in Denver, Colo., March 



4 Mycol. 2 : 248. 1910. 



5 For previous cultures see Jour. Myc. 14: 21. 1908; Mycol. 2: 224. 1910; 

 and 4 : 16. 1912. 



