302 



Mycologia 



found on the dead plants of the same annual grass, all pointing to 

 a genetic connection. Hard clumps of earth with the young 

 grass were taken at some distance from the aecial areas and 

 brought to the greenhouse at Lafayette. Whole plants of the 

 Allium, in size and appearance much resembling the garden onion, 

 on which were abundant aecia, were also taken, and on June 6 a 

 sowing was made on the seedling grass,, resulting in uredinia, 

 first noticed June 21. Another lot of Allium plants from, the 

 same field, bearing aecia, was sent by Mr. Melchers about two 

 weeks later, and another sowing made June 22, giving uredinia 

 June 29. Neither cultures developed telia, owing to the diffi- 

 culty in keeping the grass growing properly in the greenhouse. 

 No uredinia appeared on any of the grass not used for inoculation. 



The first cultural attempt with the telia of this rust was made 

 fourteen years ago, 10 and again in 1910 and 191 2, many hosts 

 being used. 11 In 1912 the attempt was made to follow up ob- 

 servations by Mr. E. Bartholomew at Stockton, Kans., by sowing 

 on Allium reticula'tum, among other hosts, but with no success. 

 Again this year Mr. Bartholomew sent telia on Sporobolus 

 neglectus, which were sown on A. reticulatum and A. canadense, 

 but again without success. No reason can be assigned for the 

 failures. 



2. Puccinia Vernoniae B. & C. — A collection of this rust on 

 dead stems of some undetermined species of Vernonia, obtained 

 by Mr. C. H. Crabill at ClifTview, Va., March 9, was forwarded 

 by Dr. F. D. Fromme. The sori were numerous and large, rang- 

 ing from 0.5 to 1.5 cm. long. A sowing was made on the leaves 

 of an undetermined species of Vernonia, which resulted in an 

 abundance of pycnia, and some uredinia, but growth ceased 

 before telia were formed. Exact dates were not secured, but the 

 sowing was made about April 30, and the leaves removed for the 

 herbarium on July 6, bearing pycnia and uredinia. This is the 

 first record for pycnia of this very common rust. The life cycle 

 shows that the species belongs in the genus Bullaria, in which it 

 becomes Bullaria Vernoniae (B. & C.) comb. nov. 



10 Bot. Gaz. 35: 11. 1903. 



11 See Mycol. 4: 13. 1912; and 7: 66. 1915. 



