Cultures of Uredineae in 1916 and 1917 307 



Houstonia caerulea, and since then efforts have been made to 

 secure suitable material with which to make tests. With this in 

 view teliospores from a collection on Aristida ramosissima made 

 by Prof. H. S. Jackson at Elberfield, Ind., were sown on H. 

 caerulea, but without result. Again teliospores from a collection 

 made by Prof. C. D. Learn on A. basiramea at Stillwater, Okla., 

 were sown May 24 on H. caerulea, Plantago Rugelii, P. lanccolata 

 and P. aristata, with infection only on the last two hosts, giving 

 pycnia June 4 and aecia June 7, both in abundance. 14 



4. Uromyces perigynius Halst. — Aecia have been collected on 

 Rudbeckia laciniata in the central west from Montana to Wis- 

 consin, Nebraska and Indiana for which telial connection has been 

 sought for some time. 15 Field observations made by Prof. E. W. 

 D. Holway at Excelsior near Minneapolis, Minn., during 1916, 

 pointed definitely to telia on a Car ex. The material sent by Prof. 

 Holway was U. perigynius on C. sparganioides, and was sown 

 May 17 on Solidago canadensis, Aster lateriflorus and Rudbeckia 

 laciniata, with exceedingly abundant infection on the last host, 

 showing pycnia May 21 and aecia May 29. The Solidago and 

 Aster remained free of rust. Evidently Rudbeckia acts as a 

 racial host for this rust parallel with Aster and Solidago. 



Successful cultures in 191 7 reported now for the first 

 time. — Both of the following species introduce questions of rela- 

 tionship which it is impossible at the present time fully to answer. 



1. Puccinia Sporoboli Arth. — Observations were made by 

 Dr. J. F. Brenckle at Kulm, N. Dak., which pointed distinctly to 

 the connection of this rust with aecia on Lilium umbellatum, and 

 material consisting of telia on Sporobolus heterolepis and bulbs of 

 L. umbellatum were sent in the fall of 191 6, with which to test 

 the matter. Further telial material was sent the following spring. 

 A sowing was made April 26 and again April 30 and May 1 on a 

 garden lily, L. elegans Thunb., closely resembling L. umbellatum, 

 the Dakotan plant not being in leaf, but with no infection. On 

 May 16 two sowings were made on the same host and also on 



1 4 For previous cultures on Plantago see Bot. Gaz. 35: 17. 1903. 



!5 For unsuccessful tests see Jour. Myc. 12: 12, 13, 14, 25. 1906; 13: 191, 

 192, 202. 1907; Mycol. 1: 229, 230, 251. 1909; 2: 218. 1910; 4: 9, 12, 13, 28. 

 19 12. 



