102 



Mycologia 



is a red pigment produced in some cases, but most cultures re- 

 main perfectly white (Fig. 2). 



Glycerin solutions. Harz (1890) described Physomyces 

 heterosporus from the surface of glycerin vats in a soap factory. 

 Here, as well as in the laboratory, it grew on solutions containing 

 as much as 30 per cent, of glycerin. To determine the ability of 



Fig. ,2. Colonies of Monascus piirpureiis one month old in silage decoction. 



the organism in question to use glycerin, flasks containing 100 c.c. 

 of 5, 10, 20 and 40 per cent, glycerin in tap water were inoculated 

 with pure cultures of Monascus. Growth occurred in the 5 and 

 10 per cent, solutions, but little or none in the 20 and 40 per cent, 

 solutions. In the 5 per cent, solution small masses of mycelium 

 could be observed within a few days, floating in the liquid. 

 These continued to enlarge slowly for two months, at the end of 

 which time they formed a semi-transparent mass of a quarter of 

 the volume of the medium. The interior of such masses was 



