Arthur: Cultures of Uredineae in 1909 223 



sown May 19 on Samhucus canadensis and Onagra biennis, giv- 

 ing no infection on the former, but giving very abundant pycnia 

 on the latter May 28, followed by aecia June 6. 



Four collections on the same host, made by Dr. J. F. Brenckle, 

 at Kulm, N. D., were used for three successful sowings on 

 Onagra biennis, giving rise to pycnia in seven to twelve days, 

 followed by aecia in five to seven days more. Sowings on Gaura 

 biennis and Carduns undulatiis gave no infection. 



A collection on Carex trichocarpa Muhl., made by Mr. F. D. 

 Kern at White House, St. Louis Co., Mo., after having been 

 sown on Sambucns canadensis with no infection, was sown April 

 21 on Gaura biennis, giving rise to pycnia April 30, and aecia 

 May lO.i^ 



2. PucciNiA Caricis (Schum.) Schrot., on Car ex arisfata 

 R. Br., collected at Kulm, N. D., by Dr. J. F. Brenckle, was 

 sown April 6 on Urtica gracilis, giving rise to pycnia April 13 

 and aecia April 17, both in great abundance. A similar sow- 

 ing April 21 on Boehmeria cylindrica gave no infection. An- 

 other sowing May 4 on both hosts gave no infection on Boeh- 

 meria cylindrica, but produced the richest possible infection on 

 Urtica gracilis, showing pycnia May 9, and aecia May 15. Be- 

 side adding another species of Car ex to the list of hosts supply- 

 ing culture material for this rust,^* the results indicate that 

 Boehmeria is not an aecial host. 



It may be well to point out in this connection that the usual 

 citation of Rebentisch for this species of rust is erroneous. This 

 author in his Florae Neomar(?hicae, 1804, page 356, describes 

 Puccinia Caricis as a new species in the following words : Sparsa 

 minutissima puncfiformis, capsnlis cylindraceis apice utrinque 

 attenuatis. In foUis Caricis praecocis Jacq." Only the one host 

 species is named, neither the description nor the host tally with 

 those known to belong to the rust having its aecia on Urtica, as 

 may be seen by consulting Sydow's Monographia Uredinearum. 

 They, however, do agree well with those given by Sydow under 

 Puccinia silvatica, to which species Rebentisch's name should 

 doubtless be referred. 



For previous cultures see Bot. Gaz. 35: 13. 1903; Jour. Myc. 8: 55- 

 1902; 11: 58. 1905; 12: 15. 1906; 13: 195. 1907; and Mycol. i: 233. 1909. 



"For previous cultures see Bot. Gaz. 29: 270. 1900; 35: 16. 1903; Jour. 

 Myc. 8: 52. 1902; 12: 15. 1906; and 14: 14. 1908. 



