Arthur: Cultures of Uredineae in 1909 225 



5. PucciNiA SUBNITENS Diet., Oil DisticJiHs spicata (L.) 

 Greene, collected at Kulm, N. D., by Dr. J. F. Brenckle, was 

 sown April 23 on Corydalis aiirea Willd., with no infection, and 

 also on Atrip! ex hastata, giving pycnia May 3, and aecia May 12. 

 Another sowing was made April 24 on Corydalis sempcrvircns 

 (L.) Pers. (C. glauca Pursh), with no infection, and also on 

 Atriplex hastata and Chenopodium album, both of which gave 

 infection, showing pycnia April 31, and aecia later.^' The chief 

 object in again growing this rust was to see if it would infect 

 species of Corydalis on which occur Aecidimn fumariacearum 

 Kellerm. & Sw. {A. Corydalis Webber), the aecia and aeciospores 

 of which resemble those belonging to Puccinia suhnitcns. The 

 results, however, are inconclusive, and further cultures must be 

 attempted before the question can be settled. 



6. Puccinia amphigena Diet,, on Calamovilfa longifolia 

 (Hook.) Hack., collected at Kulm, N. D., by Dr. J. F. Brenckle, 

 was sown May 22 on Smilax hispida, and gave numerous pycnia 

 May 31, and aecia June 4. This extends the geographical range 

 from which material has been received for successful cultures. 



7. Puccinia fraxinata (Schw.) Arth., on Spartina cynosu- 

 roides Willd., collected at Kulm, N. D., by Dr. J. F. Brenckle, 

 was sown May 22 on Fraxinus lanccolata, giving rise to pycnia 

 May 29, and aecia June 4.^^ 



8. Puccinia Phragmitis (Schum.) Korn., on Pliragmites 

 communis Trin., collected*at Scotia, Neb., by Rev. J. M. Bates, 

 was sown May 14 on R u mex crisp us, giving rise to pvcnia May 

 22, and aecia May 29, both in the greatest abundance. Another 

 collection by the same person, made at Grand Island, Neb., was 

 sown May ly on R. crispus, giving abundance of pycnia May 23, 

 and aecia May 29.-° 



9. Puccinia obliterata Arth., on Agropyron sp., collected 



"For previous cultures see Bot. Gaz. 35: 19. 1903 ; Jour. Myc. n : 54. 

 1905; 12: 16. 1906; 13: 197. 1907; 14: 15, 1908; and Mycol. i: 234. 1909. 



"For previous cultures see Bot. Gaz. 35: 20. 1903; Jour. Myc. n: 57. 

 1905; 12: 16, 1906; and 14: 15. 1908. 



For previous cultures see Bot. Gaz. 29: 275. 1900; Jour, Myc. 11: 57. 

 1905; 12: 16. 1906; 14: 14. 1908; and Mycol. i: 236. 1909. 



For previous cultures see Bot. Gaz. 29: 269. 1900; Jour. Myc. 9: 220. 

 1903 ; and 14 : 15. 1908. 



