226 



Mycologia 



August 15, 1908, at Granby, Colo., by Mr. E. Bethel, was sown 

 April 21 on Thalictriim alpimim and T. dioicum. There was no 

 infection on the latter host, but on the former it was abundant, 

 showing aecia May 6. Pycnia were so scantily produced that it 

 required an extended search, aided with sections from the affected 

 spots to discover any. A careful morphological study reveals 

 no difference between the telial material used for this culture 

 together with the aecia produced by it, and the telial material on 

 the same host used last year, together with the aecia then pro- 

 duced on Aqiiilegia.-^ The gross appearance of the infected 

 areas, the manner of the hypertrophy, and the remarkable pau- 

 city of pycnia, also combine to indicate that the two cultures be- 

 long to one species, although the production of aecia on both 

 Thalictrinn and Aquilegia by a single species is not in accordance 

 with similar studies made in Europe. 



10. PucciNiA MuHLENBERGiAE Arth. & Holw., on Muhleii- 

 bergia glomcrata Trin., collected at Stockton, Kans., by Mr. E. 

 Bartholomew, was sown May 17 on two plants of Callirrhoe in- 

 volucrata, both giving rise to numerous pycnia May 31, and to 

 aecia June 6 in one case and June 12 in the other. At the same 

 time it was sown on Napaea dioica, Althaea rosea and Sidalcea 

 sp., with no infection. These hosts are known to bear aecia of 

 the same appearance and morphological structure as those se- 

 cured by the culture on Callirrhoe, and the reason why they were 

 not affected by the sowings is not clear, unless this species of rust 

 is made up of races. 



11. PucciNiA Impatientis (Schw.) Arth., on Elymus striatus 

 Willd., collected at Lafayette, Ind., by Mr. A. G. Johnson, was 

 sown Alay 6 on Napaea dioica, with no infection, and at the same 

 time on Iinpatiens aurea, giving rise to pycnia May 15, and aecia 

 in abundance May 25. Another collection on the same host, made 

 by Mr. F. D. Kern at White House, St. Louis Co., Mo., was 

 sown May 11 on 7. aurea, producing pycnia May 21, and aecia 

 May 29. It was also sown at the same time and produced no 

 infection on Napaea dioica, Callirrhoe involucrata, Thalictriim 



See Mycol. i : 250. 1909. 



See Klebahn, Wirtsw. Rostpiize 275, 276. 1904. 

 For previous cultures see Mycol. i: 251. 1909. 



