Arthur: Cultures of Uredineae in 1909 



227 



dioicum, Actaea alba, Caidophyllum thalictroides, Boehmeria cy- 

 lindrica, Myosotis palustris, Mimulus ringens, Polemonium rep- 

 tans, Hydrophylluni virginicum, Poly gala Senega, Dirca palus- 

 tris, Psoralea Ouobrychis and Ipomoea pandurata. 



Aeciospores from Impatiens aiirca Muhl., were sown on three 

 species of Elymus. For this purpose small plants bearing aecia 

 were taken from the field, where they grew close together, and 

 had presumably received infection from one source. These were 

 established in pots, and the pots adjusted over the plants of 

 Elynius to be infected, so the spores would drop of themselves 

 upon the leaves of the grass, belljars being used as usual to secure 

 the right degree of moisture for the germination of the spores. 

 In this manner aeciospores were sown June 4 upon Elymus znr- 

 giniciis L., E. canadensis L. and E. striatus Willd. In all three 

 cases uredinia began to show June 17, and continued to increase 

 for some time, but the conditions were not such as to keep the 

 plants in healthy condition for the maturing of telia. 



Former cultures-* had demonstrated the genetic connection be- 

 tween the aecia on Impatiens and telia on Elymus virginicus. 

 The present season's work indicates that the rust on all species of 

 Elymus in the region east of the Rocky Mountains belongs to one 

 species, Puccinia Impaticntis. 



12. Puccinia poculiformis (Jacq.) Wettst., on Agropyron 

 pseudorcpens Scribn. & Sm., collected at Kulm, N. D., by Dr. J. F. 

 Brenckle, was sown May 4 on Berheris vulgaris, giving rise to 

 pycnia May 12, and numerous aecia May 24. Another collection 

 on Sitanion longifolium J. G. Sm., made at Tolland, Colo., 9,000 

 feet altitude, by Mr. E. Bethel, was sown May 17 on Berheris 

 ■vulgaris, and gave pycnia May 29, but owing to maturity of the 

 leaves did not reach the production of aecia. Still another collec- 

 tion on Sitanion longifolium, made at Eldorado Springs, Colo., 

 4,500 feet altitude, by Mr. E. Bethel, was sown April 26 on Ber- 

 heris vulgaris, and gave pycnia May 3, and aecia May 12, 

 both in great abundance. On May 17 the barberry plant bear- 

 ing aecia, produced in the last culture, was arranged over a 

 pot in which young wheat plants (Triticum vulgar e Vill.) were 

 growing, in such a manner that the aeciospores could fall 



See Bot. Gaz. 35: 18. 1903; Jour. Myc. 10: 11. 1904; and 11 : 57. 1905. 



