36 



Mycologia 



media in plates and pure cultures were again obtained. In the 

 winter of 1910-11, these cultures from ascospores were used to 

 inoculate cotton bolls on plants in the greenhouse. These bolls 

 became af¥ected and developed the pycnidia and conidia. Other 

 inoculations made direct from bolls covered with the pycnidial 

 stage have developed the perithecial stage later in the season. As 

 a result of these inoculations, the identity of the two stages is 

 proven. 



From this study, it seems questionable whether the Botryo- 

 sphaeria that we have on cotton bolls in the south is really the 

 same as the one that is so common on a large number of woody 

 plants in all parts of the country. In a paper read before the 

 American Association for the Advancement of Science in Decem- 

 ber, 1909, Shear produced evidence to show that the common 

 form of Botryosphaeria fuliginosa is connected with a pycnidial 

 stage with spores usually colored and sometimes septate, a stage 

 that could belong to either Sphaeropsis or Diplodia. As the 

 Botryosphaeria which we have on cotton bolls in the south is not 

 connected with a Diplodia, it would look as if the cotton Botryo- 

 sphaeria is specifically distinct from the common form. 



Louisiana Agricultural Experiment Station, 

 Baton Rouge, La. 



