Fraser: Cultures of Heteroecious Rusts 73 



tion, but the plants did not flourish. A pot of Atriplex obtained 

 at the same place as the others was kept as a control ; it remained 

 free from aecia. 



A number of plants of Atriplex had also been transferred to 

 the garden outside. A sowing was made on these by germinat- 

 ing the teliospores in a moist chamber, and suspending the Dis- 

 tichlis leaves bearing them so that the basidiospores would fall on 

 the Atriplex plants ; the whole was then covered with a bell jar for 

 about two days. This sowing was made on May 28 and on June 

 11 abundant pycnia were noticed, which probably appeared before 

 that date, as the plants were not carefully watched. Aecia began 

 to form in abundance but they were eaten out of the leaves, prob- 

 ably by insect larvae or slugs. The experiment was carried on 

 outside. 



The infection was so marked that it left little doubt that the 

 forms were connected, and to establish this another experiment 

 was tried in the laboratory. Plants of Atriplex were obtained 

 as before; also a number of flourishing young plants of Cheno- 

 p odium album L. from a waste heap. Sowings were made on 

 these on June 12 by suspending above them Distichlis leaves bear- 

 ing telia that had been germinated in a moist chamber. The 

 whole was then covered as usual with a bell jar for a day or two. 

 The plants were carefully watched and pycnia became evident on 

 June 21 and were soon followed by abundant aecia. The infec- 

 tion was very marked on both the Atriplex and Cheno podium, 

 but especially so on the latter, where the aecia frequently formed 

 rows on the leaves corresponding to the Distichlis that had been 

 suspended above. No aecia developed on the plants in the field 

 around those that had been used for the experiments, and controls 

 of Atriplex remained free from infection. Some of the Atriplex 

 plants used in the experiments were matured and were found to 

 be Atriplex patula var. hastata (L.) Gray. 



Collections of aecia were made on Salicornia euro pea L. and 

 Suedia maritima (L.) Dumort, which, from their association with 

 the telia on Distichlis, seemed to be also connected. A sowing 

 was tried on Salicornia, but without result. The plants, however, 

 did not flourish and it may have been on that account that there 



