Buchanan: Morphology of Cephalosporium 171 



das durch Schleim zusammengeballte kuglige Konidienkopfchen. 

 Dagegen zeigen die Konidientrager in trockner Luft nur einzelne 

 Konidien welcher locker zu Kopfchen zusammentreten. In letz- 

 teren Falle zeigt sich gegen Cephalosporium nicht der geringste 

 Unterschied, so dass ich der Meinung sei mochte, Hyalopus ist 

 nur ein unter feuchten Verhaltnissen wachsendes Cephalosporium. 

 Weitere Beobachtungen uber die Bildung der Konidienkopfchen 

 waren sehr erwiinscht." 



The species and variety of Cephalosporium here described were 

 isolated from humus-rich soil on dextrose agar made up without 

 intentional addition of combined nitrogen in any form. The colo- 

 nies develop quickly on a variety of media, peptone agar and 

 gelatin, mannite and dextrose N free agar, mannite dextrose and 

 starch N free solutions, peptone solution, peptone and dextrose 

 gelatin and solidified blood serum. The rapidity, type and luxu- 

 riance of growth exhibit some differences on the various media 

 employed. 



The mycelium is in all cases hyaline, at least when young, septate 

 and much branched. The sterile hyphae are of indeterminate 

 length. On dextrose agar plate cultures they grow from center to 

 circumference of the Petri dish in the course of four days to a 

 week at 20 0 C. The diameter of the hyphae varies from 5 to 

 25/x. The cell contents, at first homogeneous, become somewhat 

 vacuolate and later hold a large number of oil drops. There is 

 little evidence of negative chemotropism of the hyphae toward 

 each other, as they cross and recross repeatedly. The hyphae 

 penetrate the medium to a depth of half an inch in agar tubes. 

 The organism grows well only in the presence of an abundance 

 of oxygen. Aerial hyphae are not thrown off in abundance 

 from the surface of a plate culture, but whenever the organism 

 comes in contact with a foreign substance as the glass wall they 

 are sent up in abundance. Some of these may reach a length of 

 half an inch or more. 



The conidiophores are developed abundantly on all hyphae that 

 lie at the surface of the medium and upon the aerial hyphae when 

 formed. A few develop even below the surface of the medium. 

 They are slender, hyaline, and vary in length from a micron or 

 two to twenty or thirty on some aerial hyphae and 10 to 50 /x on a 

 moist surface or in a moist atmosphere. They are usually non- 



