184 



Mycologia 



10.02 A.M. Paralyzed. 



10.15 A.M. Apparently recovered. 



10.20 A.M. Paralyzed again. 



10.27 A.M. Heart stopped in diastole. 



The remainder of the available air-dry specimens (20 grams) 

 was extracted according to Harmsen's scheme and the residue thus 

 obtained was subjected to the alkaloidal separation process already 

 outlined. A small amount of a waxy residue was the outcome 

 of this treatment. This residue weighed 0.07 gram. It was dis- 

 solved in 10 c.c. of water. This solution also responded to the 

 test for cholin. 



Experiment 3. August 11, 1910. Frog 11. Weight, 29 grams. 

 Received an injection of 1 c.c. of extract at 4.27 P.M. 

 4.31 P.M. Completely paralyzed. 

 4.35 P.M. Heart stopped. 



Experiments with Inocybe infida. First Group. Twenty 

 grams of pulverized Inocybe infida were extracted in the usual 

 way and the final residue, weighing 0.21 gram, was dissolved in 

 10 c.c. of water. The residue was greenish and semi-crystalline. 

 Its solution was slightly alkaline to litmus. This extract was not 

 subjected to the alkaloidal separation process. 



Experiment 4. July 15, 1910. Frog 8. Weight, 35 grams. 

 Received an injection of 1 c.c. of Inocybe extract at 2.30 P.M. 



2.37 P.M. Lethargic. Swallowing motions. 



2.55 P.M. Lethargic. Swallowing motions. Began to appear 

 bloated. July 16, 10.30 A.M. Heart stopped. Animal bloated. 



Experiment 5. July 15, 1910. Frog 9. Weight, 26 grams. 

 At 3.56 P.M. received an injection of 2 c.c. of the extract admin- 

 istered to the frog in Experiment 4. 



4.01 P.M. Nearly paralyzed. 



4.17 P.M. Wholly paralyzed. 



4.21 P.M. Heart stopped. 



Forty grams of pulverized Inocybe infida were extracted and 

 treated in the usual manner. The residue, which was waxy and 

 greenish in color, weighed 0.36 gram. It was dissolved in 20 

 c.c. of water. This solution contained cholin, as was indicated 

 by the periodide test. The alkaloidal separation process was not 

 applied to it. 



