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Mycologia 



pearance. The final residue was greenish and weighed 0.13 gram 

 The test for cholin was positive. The solution was neutral to 

 litmus. 



Experiment 10. February 13, 191 1. Frog 18. Weight, 24 grams. 

 Received an injection of 1 ex. at 2.07 P.M. 



2.25 P.M. Excited. 



2.32 P.M. Partly paralyzed. 



2.35 P.M. Completely paralyzed. 



4.30 P.M. Lethargic and partly paralyzed. 



February 14, 9.15 A.M. Apparently normal. 



Experiments with Clitocybe multiceps. Having already 

 studied the action of extracts of the poisonous Amanita mus carta 

 and the questionable Inocybe infida, we performed similar experi- 

 ments with comparable extracts of Clitocybe multiceps, which is 

 known to be harmless. This plan was intended to serve as a " con- 

 trol " of our previous procedure and to show whether we had in- 

 troduced poisonous material into our extracts during their prep- 

 aration. About 50 grams of dried Clitocybe multiceps were 

 treated in the usual manner and finally 0.23 gram of a waxy 

 residue was obtained. This residue was dissolved in 10 c.c. of 

 water. The solution seemed to be slightly alkaline to litmus. 

 The test for cholin was very pronounced, in fact, it was the most 

 striking of any yet observed by us in this study. 



Experiment 11. October 14, 1910. Frog 13. Weight, 35 grams. 

 Received an injection of 1 c.c. of the extract at 9.47 A.M. 



10 A.M. No symptoms. 



12 M. Normal and continued so. 



Experiment 12. October 14, 1910. Frog 14. Weight, 25 grams. 

 At 10.50 A.M. received an injection of 2 c.c. of the extract admin- 

 istered to the frog in Experiment 11. 



11.25 A.M. No symptoms. 



12.00 M. Slightly lethargic ( ?). 



12.45 P.M. Normal. 



October 15, 9.10 A.M. Normal and continued so. 



The remainder of the extract prepared for use in Experiments 

 11 and 12 was treated according to DragendorrT's method for the 

 separation of alkaloids. The resultant residue weighed 0.05 gram. 

 It was dissolved in 10 c.c. of water. The solution was slightly 



