Clark-Kantor : Toxicological Experiments with Fungi 187 



alkaline to litmus. Apparently there was no cholin in it, for there 

 was no response to the Rosenheim test. 



Experiment 13. October 24, 1910. Frog 15. Weight, 28 grams. 

 Received an injection of 1 c.c. of the extract at 4.07 P.M. 



4.17 P.M. Normal. 



5.05 P.M. Normal. 



October 25, 9.05 A.M. Normal. 



Experiment 14. October 28, 1910. Frog 13a. Weight, 32 

 grams. At 2.28 P.M. received an injection of 1 c.c. of the extract 

 administered to the frog in Experiment 13. 



3.02 P.M. Normal. 



3.25 P.M. Normal. 



4.50 P.M. Normal. 



Experiment 15. October 28, 1910. Frog 20. Weight, 35 grams. 

 At 4.50 P.M. received, per os, 1 c.c. of the extract administered 

 to the frog in Experiments 13 and 14. 



5.05 P.M. Excited (?). 



5.20 P.M. Normal. 



5.50 P.M. Normal. 



SUMMARY OF CONCLUSIONS 



Inocybe infida, when subjected to processes of extraction and 

 purification that separate muscarin from Amanita muscaria, yields 

 material which exerts definite toxic effects upon frogs. These 

 effects are quite different from those produced by muscarin 

 as obtained, by the same method, from Amanita muscaria. A pro- 

 longed state of lethargy, often with complete recovery after twelve 

 or fifteen hours in this condition, was a constant factor in our 

 toxicological experiments with this Inocybe. The poison seems 

 to be of the narcotic type recently found by Ford in Inocybe 

 infelix. 



The poison of Inocybe infida seems to belong chemically to the 

 class of alkaloids or related substances. The plants of this spe- 

 cies are small. A very much larger supply of these mushrooms 

 than the available quantity will be required for the isolation and 

 chemical identification of the toxic material. 



Dr. William A. Murrill called Professor Gies' attention to the 



