CONTRIBUTIONS FROM THE WISLEY LABORATORY. 395 



The organisms are thus widely distributed in the Wisley soils and 

 exist in places where it seems difficult to account for their presence, e.g. 

 in cultures Nos. 104 and 105. This only bears out observations made in 

 gardens and fields, where it is extremely rare to find leguminous plants 

 without nodules. The French beans lacked nodules more frequently than 

 the other plants, but in all the land that had been under cultivation they 

 were found. 



Inoculation of Non-Leguminous Chops. 



Both in 1908 and 1909 attempts were made to test the influence of 

 Nitro-Bacterine when used for non-leguminous crops. 



In 1908 cabbages and lettuce, and in 1909, beet and tomatos were 

 planted on the poor fallowed land treated with powdered chalk, some 

 being inoculated and some not, but in no case was satisfactory growth 

 made. The inoculation was evidently incapable of overcoming the 

 poverty of the soil, and apparently of supplying nitrogen to the plants 

 put into it so as to enable them to grow properly. Further, in 1909, 

 the culture failed to become very cloudy within the forty-eight hours 

 although kept under the very best conditions for development, indicating 

 that it was at least weak, and showed itself to be contaminated with 

 considerable numbers of organisms other than nitrifying ones. 



In the case of the tomato, the individual differences between the 

 plants was so great as to render any comparison between the growth in 

 the adjacent rows of very doubtful value. 



Eadishes were grown in pots under glass in the same poor soil and 

 supplied with lime. Under these conditions the inoculated plants gave 

 a yield about equal to that of the uninoculated. 



It may be added that manuring with nitrogenous manures such as 

 nitrate of soda and nitrate of lime resulted in obtaining average crops of 

 both turnips and radishes in this soil. 



