127 



generation on the very day after inoculation. The colony on the 

 potato medium Avas composed of very small and short bacilli, 

 smaller than the tubercle bacilli ; they performed, in a hanging 

 drop, an active vibratoy movement, and were not easily stained 

 with Locfflcr s blue, but better with ZiehV s solution : they were 

 very liable to undergo involution, soon assuming a dumbbell 

 or diplococcen-like, form, after which the staining became 

 much more difficult. In the pus of an inoculated guinea 

 pig they aj^peared larger and longer, even longer than the 

 tubercle bacilli, and afforded, when stained, a charateristic 

 interruption of colour. This microbe was not cultivable directly 

 from the horse, but always through the medium of experimental 

 animals, and was pathogenic to the guinea pig and horse, as seen 

 in the following experiment : 



Inoculation of guinea pigs witJi pure culture of tlie bacilli ; 

 the result was as follows ; — 



Male guinea pig No. 28, inoculated on August 7th, '88, 

 showed on the next day strong local inflammatory reaction, the 

 praeputium, scrotum, &c. being swollen, reddish, and painful, 

 and a bloody purulent discharge from the wound. These acute 

 symptoms gradually subsided, while the general condition was 

 getting worse day by day. On the 8th day after inoculation, the 

 inguinal gland was swollen to pea size, and three nodules, and 

 an ulcer appeared on the scrotum. On the 13th day the right 

 carpal joint was found swollen, reddish, and painful, and another 

 small ulcer appeared on it ; afterwards the swelling became 

 larger. On the l6th day the animal was destroyed and dissected: 

 a small abscess in the scrotum, and an induration of para-articular 

 tissue of the right carpus, with collateral oedem in the surround- 

 ing tissue ; the parenchym of testicles, lymphatic glands and 

 internal organs free from affection. 



Male guinea pig No. 29, inoculated on August 7th, '88. 

 Acute symptoms in the ist stage similar to No. 28. On the 

 8th day after inoculation a pea-sized ulcer was found at loc2is 

 inoculationis; on the 9th day the animal was destroyed ; the 

 postmortem revealed only local ulceration, no internal change. 



Male guinea pig No. 30, inoculated on August 7th, '88, 

 showed the same symptom in the beginning. On the 8th day 

 after inoculation 2 small abscesses at the sheath of penis and 



