128 



scrotum were found ; on the 9th day the animal was destroyed and 

 dissected, and the postmortem was similar to No. 29. 



Male guinea pig No. 31. Inoculation-date and the first 

 symptoms were the same as in the preceding. On the 8th 

 day a dry ulcer appeared at the loais inocu/ationis ; on the 13th 

 day both carpal joints, scrotum and the sheath of penis swollen, 

 and the latter partly fluctuating ; on the l6th day an ulcer 

 appeared on the dorsal surface of carpus. The postmortem 

 was nearly the same as in No. 28. 



Male guinea pig No. 32. Inoculation-date same as the 

 preceding. Reaction was similar to No. 29. 



From the pathological products of the above mentioned 

 guinea pigs, we obtained always a pure ciilttire of the same 

 bacillus as the original 



Inoculation of experimental horses zvith pure culttire : — A 

 stallion of Japanese breed No. 3, 3 years old, quite healthy, was 

 inoculated, on August 9th 7 a.m., with pure culture, in two places, 

 in the subcutis of the right shoulder and in the mucous membrane 

 on the right side of the septum nasi. On the next day temperature 

 38.3^, pulse 64, respiration 34-35, jirofuse purulent discharge from 

 the right nostril ; inoculation place of the right shoulder was 

 swollen to the size of an apple, but the horse was still active. 

 On the 3rd day temp. 40.6°C., pulse and resp. also accelerat- 

 ed ; profuse purulent discharge from both nostrils ; subcutaneous 

 swelling increasing, and both intermaxillary glands a little 

 swollen. On the 4th day temp. 39.3°C., and the scarified wound 

 of nose changed to a big ulcer, 3 cm. in diameter. On the 

 5th day temp. 39.3^0., pulse 56, resp. 11; the subcutaneous 

 swelling smaller and flatter, and the inoculation — wound gaping 

 covered with yellow scabs ; nasal discharge lessened. On the 

 6th day temp. 39.2°C. ; on the 7th day temp. 39°.C., both in- 

 termaxillary glands enlarged to thumb-size, but still dislocable. 

 In the whole course the horse showed lessened appetite and 

 fell gradually in his condition, and at last, on the 8th day, the 

 horse was destroyed for dissection. Autopsy : ulcerative destruc- 

 tion of the Schneiderian membrane on both sides of septum 

 nasi, and swelling (medullary) of intermaxillary gland ; internal 

 organs free from changes. 



Young stallion of Japanese breed No. 4. Inoculated also on 

 August 9th 7. a.m., on the right side of nose and on the right 



