141 



yellowish pus. Both testicles contained a number of mcduallary 

 herds. 



Microscopicall}' we found numerous saccharoviyccs in every 

 morbid product and tissue. In the lymphatic glands they were 

 abundantly found in the medullary swelling, while in fhe hyper- 

 plastic portion they were scanty. Long diameter of the microbe 

 4.0 fi. and the transverse 3.6 //.. It contained a granule 

 of the diameter 0.5 u.. The microbes were partly free and 

 jiartly contained in pus corpuscles, measuring 13-21 /a Most 

 of the microbes did not stain with common aniline dyes, or 

 with haematoxlin, carmine, &c., but were easily stained with 

 picric acid and iodine ; the granules and those saccharomyces, 

 full of protoplasm and perhaps younger ones, could be easily 

 stained with aniline dyes. Besides this we found, in the inter- 

 maxillary abscess, a few diplococci and streptococci. 



Culture experiment : — The content of inguinal abscess was 

 cultured on and in the following media, — potato slices, nutrient 

 gelatine, glycerine-agar, and beef bouillon. Until the 22nd 

 day there was no apparent vegetation. Microscopically we 

 found in the gelatine and agar media soine saccJtaroviyccs, 

 which were swollen to larger spherical corpuscles, nearly 

 double the original size, — 6-7 //. in diameter. These swollen 

 corpuscles contained a number of granules, 2 in dia- 

 meter. We watched for a longer time, but no furihcr changes 

 were observed. In simple, not neutrallized horse bouillon, 

 appeared on the next day flocculent precipitate of streptococci 

 and after a month wc found among the swarm of streptococci 

 some saccharomyces which were spherically dilated. 



Inoculation : — 3 guinea pigs (No. 78-80) were inoculated on 

 February 22 with farcy matter ; 2 of them showed locally a 

 pea-sized abscess, which turned to an elongated ulcer. The pus 

 from the abscess contained numerous saccharomyces in asso- 

 ciation with different bacteria and those pus corpuscles, which 

 contained the former were often supplied with protoplasmic feet in 

 the form of pseudopodia. In one guinea pig the ulcer soon healed 

 by cicatrization. In the other the ulcer persisted for a long- 

 time, and the animal died on March 13 from plcuro-pneumonia ; 

 in the pleural exsudation and diseased ])art of the lungs, we 

 could not find any saccharomyces. In the third guinea pig there 

 wa.s no proper reaction. 



