149 



varicose hyphen containing differing numbers of granules. On 

 glycerinc-agar ap,d not-neutralized-pepton-agar media kept in a 

 thermostat, we noticed, after 30 days, the vegetation of saccJia- 

 roniyces in tlic form of granular colonies similar to those in 

 gelatine medium. On the 43rd day, the colony attained the size 

 of 1-4 mm., distinctly prominent and irregularly wrinkled. On the 

 coagulated blood serum from the horse, v.'ith or without glycerine, 

 the vegetation was feeble ; on the potato medium vegetation 

 appeared first on the 29th day ; tl.c colony was nearly the same 

 as agar-colony. In every occasion slightly acid media were 

 more favorable for the vegetation than the alkaline. Dimen- 

 sions of the vegetative saccliarouiyces were as follows : — 



Lonsit. diam. Tiansv. tliam. 



Little grown cell 2.50-3.652 //. 1.66-2.50 



Medium grown cell ■ • • 6.64 4.9S 



Much grown cell 12.28 12.45* 



Hyphen form 16.66- 19.92 



Granules (largest) 3.486-5.000 



ditto (smallest) O.830-I.660 



Thus a vegetative form was larger by 2/3-3/4 than the 

 original size, and the maximum was even triple or fourfold. 



Inoculation with the cnltiire of snccJiaroniyccs : — An experi- 

 mental horse No. lO was twice inoculated subcutaneously with 

 pure culture, ist on Apr. i6th and 2n(l on June 14th. After the first 

 inoculation, induration and suppuration appeared locally ; after 

 the second, again local induration appeared, which persisted for 

 a long time (14/vi-lX) and gradually turned to abscess. The 

 abscess opened of itself on Oct. 20th, and in the pus we found 

 numerous saccJiaroniyces . Both lesions were circumscribed, and 

 without further changes they completely disappeared. The 

 culture was introduced in the subcutis and abdominal cavity of 

 guinea pigs and rabbits, but tlicrc was no specific reaction. 



Anaerobic cultivation in hydrogen atmosphere was negative, 

 so far as saccJiaroniyces were concerned. One kind of small 

 short bacilli, facultative anaerobe, was isolated ; this caused 

 strong suppuration when introduced in the subcutis of a horse. 



Inoculation i^.'ith morbid matter: — Two guinea pigs (No. 86-87), 

 inoculated with farcy pus, showed only local suppuration. Of 

 guinea pigs (No. 88-90) inoculated on May 15th, with morbid 

 tissue of skin, two died from malignant oedem and the 3rd 



Exceptional dimension. 



