I/O 



take the usual bacterial stains, also those granules, which 

 are free in the liquor piiris, can not be stained. 



This microbe can hardly grow on artificial media, such as 

 bouillon, nutrient gelatine, nutrient agar, potato, &c. The 

 growth is exceedingly slow, and requires a considerable space 

 of time before the vegetation becomes apparent. After a week 

 the oblong cells are dilated to larger spherical corpuscles and 

 the central granule or nucleus is also soaked, enlarged, and 

 divided into 2 or more daughter granules of faintly yellowish 

 and homogenous quality. The swollen microbe often attains 

 a diameter of 6, 7 even 12*45 /'> ^'^^^ the granule of 2-5 u.. 

 After a time the spherically swollen microbe assumes an oblong, 

 cylindrical or dumbbell form, and then by partition it is divided 

 into 2, 3 or more segments, and finally developes into a kind of 

 Jiyphcn ; tlie latter is thinner in diameter than the spherical 

 form, showing varicosities in different parts. In further course 

 of time there appear by budding a number of secondary /////^r/^^, 

 and the tertiary from the secondary IiypJicn. Meanwhile the 

 granules increase in number and accumulate especially in the 

 terminal segment of the ItypJicn. 



Upon the nutrient agar, the vegetation first becomes ap- 

 parent after 30 days in the forin of greyish white grains. After 

 40-50 days a single colony attains a diameter of 1-4 mm, and be- 

 comes distinctly prominent over the surface of the medium. In a 

 fully grown colony, the surface is wrinkled gyi'i and sulci; the 

 colony is very compact, difficult to dissect with a platinum wire 

 or to crush under a covering glass. Microscopically it consists 

 of conglomerated pil."raseii, composed of hyphen, spherical 

 cells and a great number of free granules. The addition of 

 grape sugar or glycerine to the medium has no influence on the 

 vegetation. In the nutrient gelatine the vegetation takes place 

 especially on the upper stratiiiit ; 56 days after; the colony 

 appears as a yellowish white sandy mass of an irregular shape, 

 1-3 mm. in diameter; the gelatine is not liquefied. When the 

 gelatine is accidentally molten and the colony sinks, the 

 growth is no longer continued. Ou potato slices the colony 

 looks more brownish ; otherwise it is similar to the agar-colony. 

 Fluid media may also be used for the cultivation of this microbe, 

 but pepton must be added. It does not grow in horse-dung 

 infusion, hay infusion, sugar solution, &c. The optimal tempera- 



