234 



K. VABE ; ON TWO NEW KINDS 



influence may also be observed on cultivation in Pasteur s solu- 

 tion, in wliich those cells that grow attached to the sides of the 

 vessel near the surface of the liquid, assume a fine red colour 

 before the sediment has reached this intensity. The influence 

 of light is not required, as the colour is just as well developed in 

 perfect darkness. 



This species is further incapable of forming alcohol from 

 glucose or cane sugar ; neither could any development of gas 

 bubbles in the liquid nor a trace of alcohol in the distillate be 

 observed, when the yeast was left for some time in complete 

 nourishing solutions containing those sugars. 



Cane sugar and glucose proved to be better nutrients for this 

 organism than milk sugar ; the fungoid mass grown in lO^ milk 

 sugar solutions containing o.^% meat extract, weighed under 

 equal conditions about half as much as when grown with glucose 

 or cane sugar as nutrients. A retardation of the development is 

 observed upon addition of 2% alcohol to the nourishing liquid, 

 while 7^ stops it altogether, which demonstrates a much greater 

 sensibility to alcohol than beer or wine yeasts possess. A tempera- 

 ture of 40°C kept up for 15 minutes injures the cells to some 

 extent, multiplication being retarded, but one of 45° acting for 15 

 minutes, kills the cells. 



Stab-culture in sugar-gelatine even after weeks shows hardly 

 a trace of development along the needle track, while on the sur- 

 face a red film gradually develops, producing a concavity with 

 gradual liqticf action of the gelatine. This shows the organism to 

 be exquisite aerobic, which well agrees with its inability to 

 produce alcohol from sugar. 



I have paid special attention to the question whether this 

 species would be capable of forming ascospores, and have sub- 

 jected well nourished cells after transference upon moist gypsum 

 blocks to close observation, but in no case did ascospores make 

 their appearance. The only mode of propagation is gemmation 

 which is, however, not only carried on in the usual way but also 

 in another manner heretofore not observed, as far as is known to 

 me. This peculiarity consists in the presence of a fine mycelial 

 thread by which the young cell is attached to the mother cell. 

 In gemmation many cells develop first a fine, not separated myce- 

 lial thread, at whose ends the young cells develop as shown in 

 the following figure (1). 



