238 O. LOEW AND S. TAKABAYASHI ; ON BROM ALBUMIN 



ajjplied to remove the last remnant and to obtain a colourless 

 product. This contained \6.\6% bromine but after dissolving it 

 in ammonia and precipitating by acids only 13.10^. 



This bromalbumin yielded no potasium sulphide on h.eating" 

 with a solution of caustic potassa, nor tyrosine on heating with 

 hydrochloric acid, but it did then yield leucine. It gave further 

 the biuret, but not the Milloii s reaction. It was soluble in 

 dilute alkalies and precipitated again by acids. 



We have recently prepared bromalbumin again, but with the 

 modification that equal weights of albumin and bromine were 

 taken, and the mixture (cooled during preparation) heated for 

 two days to about 6o°C. After washing with water,'" aqueous 

 sulphurous acid was applied to remove the last traces of free 

 bromine. It was then washed with, a dilute solution of sodium 

 carbonate, and treated with alcohol of 50%, until no more reac- 

 tion for bromides was obtained in the filtrate ; final 1\- with abso- 

 lute alcohol. Dried at iOO°, 0.640 g. yielded 0.1650 g. A g Br = 

 11.00°^ Br. 0.637 yielded 0.1565 g. Ag V>k.= \o.6x% Br. This 

 product contained, therefore, less bromine than that obtained 

 under the conditions described above. 



In order to observe the behaviour to microbes 12 g. were 

 dissolved in 150 cc. of a 2.% solution of crystallised sodium 

 carbonate, the solution diluted to 1200 cc. and finally 0.2% 

 dipotassium phosphate and 0.02^,'^ magnesium sulphate added. 

 To a part of this solution was then added 0.^^% peptone, and to 

 another 2% cane sugar. One half of each solution was infected 

 with microbes from putrid meat, the other -with anthrax bacilli> 

 of course under all the necessar\' precautions of sterilization- 

 Two divisions were further made to observe the effects of the 

 presence and absence of air. In the Litter case Er/etiineycr 

 flasks were provided with sterilized india rubber stoppers carrying 

 a bent tube filled with sterilized water and containing some 



(I) Th's wash water removed a considerate portion of proteid-like bodies. 

 Phospho-tungstic acid and mercuric nitrate give precipitates ; MiHoii s reaction fails. 

 Saturation witli ammonium sulph:ite yields a strong, with sodium sulphate a weaker, 

 precipitate. 



