AND ITS BEHAVIOUR TO MICROBES. 239 



mercury to prevent the ingress of air. After 24 days standing in 

 the dark at a temperature varying from 11-20°, the flasks were 

 examined with the following result : 



Microbes of Putrefaction. 



Bromalbumiii l% 



Bromalbumin 1% and 

 peptone o.S% 



Bromalbumin 1% and 

 cane sugar 2% 



In presence of air. 

 Reaction neutral. Slight 

 trace of ammonia. No 

 putrid odour, although 

 numerous cocci and 

 bacilli present. 



Reaction alkaline, much 

 ammonia, putrid odour, 

 liquid very turbid, a rich 

 vegetation of microbes 

 present. 



Smell and reaction acid. 

 No ammonia. Rich 

 bacterial vegetation pre- 

 sent. 



In absence of air. 

 Reaction neutral, no ammo- 

 nia, no turbidity, no 

 putrid odour. Mere 

 traces of cocci, but no 

 bacilli. 



Result resembling that in 

 presence of air, but bac- 

 terial vegetation less 

 developed. 



No odour, no fermentation. 

 Only slight sediment o^ 

 cocci, but no bacilli. 



Bacilli of Anthrax. 



Bromalbamin 1% 



Bromalbumin 1% 

 peptone 0.5% 



Bromalbumin 1% 

 cane sugar 2% 



ith 



with 



In presence of air. 

 Neutral, mere traces of 

 development. 



Considerable and charac- 

 teristic development. 



In absence of air. 

 Neutral. Slight traces of 

 development. 



Some development. 



Neutral ; small traces Slight traces of develop- 

 of development. ! ment. 



From these observations it follows that bromalbumin as such 

 in absence of air and even in presence of sugar is- not favourable 

 for the development of microbes. However, it does not prevent 



