250 



u. SUZUKI ; 



in the evening and not killed immediately by drying or other- 

 wise, may give rise to errors, inasmuch as the amount of proteid 

 nitrogen may be found to be lower than it was at the time of 

 gathering. The following experiments were made with the 

 intention of furnishing further support to this view. 



I. Experiment with the Buckzvheat Leaves {Polygomnn 

 f ago py runt) 



On the 8th of October the leaves of full-grown buckwheat 

 plants were removed carefully, and divided into two portions ; 

 one portion was directly dried in the air bath, while the other 

 portion was kept in the dark, the leaves being kept moist, and 

 after twenty hours, dried and analyzed. 



lOO grams of fresh leaves became by direct drying 



16.864 gram. 



100 grams of fresh leaves after keeping moist for 20 hours 



14.996 gram. 

 Ratio of dry matter lOO : 88.9 



In lOD parts of dry matter. 



Directly dried. Kept moist. 



Total nitrogen 3.90 . 4.43 



Albuminoid nitrogen 3.55 3.54 



Asparagine nitrogen 0.38 0.38 



Amido-nitrogen(;J^^°^i.) ....0.15 * 0.51 



Nitrate nitrogen 0.08 0.08 



In 100 parts of total nitrogen. 





Directly dried. 



Kept moist 





Ratio. 





100.00 



100.00 



100 



: 100 





91.00- 



79.91 



100 



: 87.8 



Asparagine nitrogen 



5-24 



8.58 



100 



: 163.7 



Amido-nitrogen(j;:'^X.)- 



1.66 



9.71 



100 



: 585 





2.10 



1.80 



100 



: 86 



