AS RESERVE MATERIAL DURING WINTER AND SPRING. 255 



When a section treated with caffeine for several hours was 

 directly treated with absolute alcohol, the globular formations 

 of proteosoines disappeared at once, while with the plasmolized 

 cells only irregular coagulated masses could be distinguished. 

 As it seemed to me that the solution of the proteosomes was 

 brought on in this case, by the caffeine being extracted more 

 quickly than the alcohol could enter, I applied to another section 

 treated with caffeine alcohol 0(20% for about 15 minutes ; whereby 

 the plasmolized cells remained partially alive and transparent, 

 Avhile the non-plasmolized cells containing the caffeine proteo- 

 somes showed then hollow or vacuolized globules, which nozu 

 on treat ment with absolute alcohol remained entirely uncJiangedP'^ 



If a section, treated for 24 hours with caffeine solution and 

 showing both plasmolysis as well as proteosomes, is treated with 

 a dilute aqueous solution of iodine in potassium iodide, we soon 

 observe the vacuolization of all the proteosomes, while the 

 vacuolized cells show irregular shaped contours of the coagulat- 

 ed cytoplasm. 



Also it is characteristic that diluted solutions of acetic or nitric 

 acid do not dissolve the caffeine proteosomes, when sufficient 

 caffeine had entered into the cells. 



The observations on the presence of active albumin in the bark 

 and buds of various plants are embodied in the following table: — 



Specie;^ Object tested Active albumin 



Larix leptolepsis | 



Present 

 Much present 



Remarks. 

 Much Starch. 



Ginko biloba 



(Buds 

 j Bark 



None 

 None 



Very much starch. 



,, no soluble proteids, no tannin. 



Rhus sylvestris j^^^,^ 



Much 

 Very much 



Much starch. 

 Little starch. 



Prunus japonica ( Bud^ 

 (Thunb) I Bark 



N'ery much 

 Very much 



Little starcli 



Prunus Grayana ( Buds 

 (Maxim) j Bark 



Much 

 Much 



Morus alba 



S Buds 

 Baric 



None 

 None 



Much starch . 



(i) In some cases, large proteosomes, commencing to develop vacuoles, considerably 

 resemble plasmolized cells, in wliich small globules of caffeine proteosomes arc developed. 

 But a close observation 'will reveal the higher refractive power of the former. 



