262 K. NEGAMI ; ON THE PHYSIOLOGICAL ACTION 



Pniiiiis triflora. 





Sodium sulphite. 



Sodium sulphate. 



Water. 



April 1 7 til. 



Twenty four buds 

 opened, but com- 

 menced to wither. 



Twenty seven buds 

 opened. 



Thirty four buds 

 opened. 



April 20tli. 



All open buds were 

 dying ; seventeen 

 buds did not open 

 at all. 



Thirty buds opened, 

 eleven were half 

 opened, the remain- 

 ing ten were not 

 opened but healthy. 



All forty two buds 

 were opened and in 

 healthy state. 



Again, on the 14th April, young branches of Brassica 

 cainpestris about 10 cm. long, bearing numerous flower buds were 

 placed in the same solutions as above. ' The development of the 

 youngest buds ceased here altogether under the influence of 

 sodium sulphite, while three of the larger buds had opened, but 

 had completely withered on the 20th April. In the two control 

 cases all the larger buds had opened and only one on each case 

 was withering, while the younger buds had increased more than 

 double in size after six days. All the chlorophyll in the young 

 leaves was destroyed by the sulphite, and also partially in the 

 case of the sulphate, probably due to the effect of the solution 

 concentrating in the leaves, while the control branches in 

 gypsum water showed the full preservation of their chlorophyll. 



III. Experiments with Leaves. 



Isolated leaves of Heliotfopitmi Perimianum and Vitis in- 

 constaiis were kept on the surface of a \% solution of sodium 

 sulphite as above. After six days they were found to be complete- 

 ly killed showing no trace of turgor at all, while tlie control 

 leaves in sodium sulphate and in water were still in a quite 

 healthy and normal state. 



