FORMATION OF PROTEIDS ETC. 491 



EVERY 100 SHOOTS CONTAIN :— 











0.0576 g 



O.O5S2 



Albuminoid nitrogen 



0.0252 



0.0305 



Asparagine nitrogen . 



0.0172 



0.0166 





0.0044 



0.000 



Other nitrogen 



0.0108 



O.OI 12 



Proteids (Alb. Nx 6.25) 



0.1575 



0.1906 





0.081 1 



0.0778 



0.0331 grams or 20^ of Proteids increased during the experiment! 



It is therefore evident that, a considerable increase of albumi- 

 noid nitrogen has taken place in the plants treated with 10% suger 

 solution ; and this increase almost coincides with the decrease of 

 nitrates nitrogen. 



II. SECOND EXPERIMENT WITH P:TI0LATED 

 SHOOTS OF BARLEY {Hordeum distichon). 



On Oct. 7, when the etiolated shoots of barley, kept in saw 

 dust, grew to the height of 15 cm., a portion of them was removed 

 from the saw dust, washed well, dried and analyzed. The other 

 portion was divided into 4 parts, and put in the following solu- 

 tions : — 



1) Half saturated gypsum solution. 



2) 0.2^ soduim nitrate, half saturated with gypsimi. 



3) 2% cane sugar half saturated with gypsum. 



4) 2% cane sugar and 0.2% sodium nitrate, half saturated 



with gypsum. 



Once in every 4 days, all these plants were transferred for a 



