FORMATION OF PROTEIDS ETC. 



(a) (b) (d) 



Dry weight of lOO shoots. 1.300 g 1.240 1.300 



EVERY 100 SHOOTS CONTAIN THEREFORE:— 





(a) 





(d) 







0.0504 g 



0.0512 



O.048S 



0.0563 





0.0264 



0.0229 



0.0229 



0.0314 



Asparaginc nitrnrren 



0.00S8 



0.0201 



0.0146 



0.0126 



Nitrates nitrogen 



0 



0 



0 



0.0013 



Other nitrogen 



0.0152 



0.0082 



o.oi 13 



O.OI 10 



Proceids (Alb. NX 6.25) 



0. 1 650 



0.1431 



0.143 1 



0.1963 



Asparasjinc (water free) 



0.0435 



0.0948 



0.068S 



0.0594 



Thus wc see that during 14 days, about IS% of nitrogen was 

 absorbed in the form of sodium nitrate, and that in the plants 

 nourished with sodium nitrate and sugar, almost all the nitrates 

 absorbed were converted into proteids ; while in the plants nourish- 

 ed with soduim nitrate alone, without addition of sugar, nearly half 

 or more of the nitrates absorbed remained unchanged on further 

 cultivation. We observe further no increase of albuminoid nitrogen 

 in the plants nourished with sugar alone. We can therefore say 

 that sugar is absolutely necessary for the reduction of nitrates for 

 plants kept in the dark. 



493 



(e) 

 1. 310 



III. EXPERIMENT WITH Phascolus imiltiflorus. 



On Sept. 22 young plants 10-15 cm. high were removed from 

 the field, carefully washed and put in a 0.2^ solution of sodium 

 nitrate half saturated with gypsum. They were kept in diffuse day 

 light for 2 days to allow them store up some nitrates. Then, one 

 portion of tliem was directly dried and analyzed, while the other 

 was again divided into 2 parts and put in the following solutions : — 



1) \% cane sugar, half saturated with gypsum. 



2) \0% cane sugar, half saturated with gypsum. 



Once in every 4 days the plants were transferred for a whole 



