494 



U. SUZUKI. 



day in solutions containing. o.i% KH2PO4. 0.1% K2HPO4. 

 0.1% MgSOi, and half saturated with CaS04. 



As the solutions were renewed every day, no bacterial turbidity 

 was allowed to appear. The plants were kept in perfect darkness 

 for 4 days/'' during which the temperature varied between ii°C. 

 and 22°C. 



Analysis after careful washing and drying gave the following 

 results : — 



IN 100 PARTS OF DRY MATTER. 





Control 

 dried on 24th 



Dark 

 1% sugar dried 

 on"28th 



Dark 

 10% sugar dried 

 on 28th 



Total nitrog;en 



5.12 



5 12 



4.07 



Albuminoid nitrogen 



2.80 



2.15 



2.12 



Asparagine nitrogen 



0.82 



1.40 



0.62 



Nitrates nitrogen 



0.36 



0.26 



0.13 



Other nitrogen 



1. 14 





1.20 



Proteids 



17.50 



1344 



13-25 



Asparagine 



3.87 



6.60 



2.92 



IN 100 PARTS OF TOTAL 



NITROGEN. 







Control 

 dried on 24th 



Dark 

 I % sugar dried 

 on 28th 



Dark 

 lo^'J sugar dried 

 on 28th 



Total nitrogen 



100.0 



100.0 



100.0 



Albuminoid nitrogen 



54-7 



42.0 



52 I 



Asparagine nitrogen 



16.0 



27-3 



15.2 



Nitrates niti-ogen 



7.0 



5.0 



3.3 



Other nitrogen 



22.3 



257 



2S.4 



We observe here a gradual decrease of nitrates nitrogen, de- 

 pending much upon the quantity of cane sugar present. But the 



(i) I could not keep them longer, as they began to show signs of suffering. 



