6 



U. SUZUKI. 



B) GINGKO BILOBA/ 0 



Fresh seeds of Gingko biloba were freed from the hard shell 

 and the juicy kernel was crushed in a mortar and extracted with 

 a 0.2-0.4% solution of caustic soda. The soda extract was 

 neutralized with dilute acetic acid and the crude proteids were 

 prepared in the same way as was described before. The prepa- 

 ration was comparatively pure and contained nitrogen. 



a). 18.775 g. air dry— 15.045 g. dry crude proteids ( = 2.2718 

 g. N.) boiled with 20% hydrochloric acid for 30 hours :■ — 



Total dissolved nitrogen. 2.2396 100.0 



Nitrogen in copper hydrate 



precipitate. 0.0372 1 .7 



Nitrogen in phospho-tungstic 



precipitate. 0.8 1 84 36.5 



Nitrogen in phospho-tungstic 



precipitate in the filtrate of 



copper hydrate. 0.75SS 33.9 



Nitrogen in ammonia. 0.2236 10.0 



Nitrogen in organic bases. 0.5356 23.9 



b). 10.143 g. air dry = 8. 135 g. dry crude proteids (=1.2285 

 g N) boiled with 0.$% hydrochloric acid for 146 hours. 



Total dissolved nitrogen. 1.2276 100.0 



Nitrogen in copper hydrate 



precipitate. 0.0372 3.0 



Nitrogen in phospho-tungstic 



precipitate. 0.4383 35.7 



Nitrogen in phospho-tungstic 



precipitate in the filtrate of 



copper hydrate. 0.4000 32.9 



Nitrogen in ammonia. 0.1265. IO -3 



Nitrogen in organic bases. °- 2 735 22.6 



(1) Recent investigation has shown that Gingko biloba does not belong to the 

 Coniferae and must be considered to form an independent family, Gingoaceae. This 

 j-lant is peculiar to Japan. 



