A CONTRIBUTION TO THE KNOWLEDGE OF ARGININ. 7 



C) PINUS THUNBERGII. 



Preparation of crude proteids in the same way as before, 

 was tolerably pure, and contained i$.6% nitrogen. 



a). 12.36 g. crude proteids (=1.928 g N) boiled for 30 hours 



with 20% hydrochloric acid. 



Total dissolved nitrogen. 1.9020 100.0 



Nitrogen in copper hydrate 



precipitate. 0.0400 2.1 



Nitrogen in phospho-tungstic 



precipitate. 0.7056 37.1 



Nitrogen in phospho-tungstic 



precipitate in the filtrate of 



copper hydrate. O.6581 34.6 



Nitrogen in ammonia. 0.1541 8.1 



Nitrogen in organic bases. 0.5040 26.5 



b)- 7-52 g. crude proteids (=1.173 g N) boiled with 0.5% 

 hydrochloric acid for 146 hours. 



Total dissolved nitrogen. 1.156 100.0 



Nitrogen in copper hydrate 



precipitate. 0.021 i.S 



Nitrogen in phospho-tungstic 



precipitate. 0.4451 38.5 



Nitrogen in phospho-tungstic 



precipitate in the filtrate of 



copper hydrate. 0.4185 36.2 



Nitrogen in ammonia. 0.0983 8.5 



Nitrogen in organic bases. 0.3202 27.7 



From the above results, we see that the organic bases are 

 splittcd off very easily by the action of dilute acid, the quantity 

 of the bases produced by the action of dilute acid is always^the 

 same with that of strong acid. 



