IO 



U. SUZUKI. 



Asparagine nitrogen. 0.1115 

 Nitrogen in phospho-tungstic 



precipitate. 0.0594 



Other nitrogen. 0.2132 



In this case, contrary to my expectation, I found very little 

 organic bases both in the etiolated shoots and in the green 

 plants. 



3. PlNUS Thunbergii. 



Seeds were sown in the purified sea sand, and kept in the 

 dark in a warm house where the temperature varied between 

 I5°C and 30°C. On the 24th February, they began to germi- 

 nate, and after 2S days they were io-i5cm. high. . They were 

 then removed from the sand, washed, dried and analyzed. 



In 100 parts of dry matter. 



Total nitrogen. 9.53 100.0 



Albuminoid nitrogen. 2.94 30.9 



Asparagine nitrogen. 2.35 24.7 



Nitrogen in phospho-tungstic 



precipitate. 1.88 19.7 



Other nitrogen. 2.36 25.7 



III. Isolation of arginin. 



I. CUYPTOMERIA JAPONIC A. 

 a). 



Isolation of arginin from the decomposition products of the 

 proteids prepared from the seeds by the action of hydrochloric 

 acid : — The hydrochloric extract was at first freed from dissolved 

 tin by hydrogen sulphide. The filtrate was then evaporated 

 off to a small volume, to expell the greater part of hydrochloric 

 acid, and diluted with water. Phospho-tungstic acid solution 

 was then added in excess, when a white precipitate was formed 

 in considerable quantities. This was collected on a filter, 

 washed with sulphuric acid until no trace of hydrochloric 



