A CONTRIBUTION TO THE KNOWLEDGE OF ARGININ. 1 5 



the basic salt was formed which after recrystallization became 

 fine needles, uniting to bundles. 



o. 113 g dry crystals yielded on ignition 0.0345 g = 30.53%Ag. 

 Calculated as C 6 H 14 N 4 0 2 , AgN0 3 +JH 2 0 = 30.55% Ag. 



We may now safely conclude that the base was arginin. 



I did not calculate how much arginin was present in the 

 decomposition products of the proteids, yet I am sure that the 

 greater part of the bases was arginin. It is not yet decided 

 whether some other bases can be isolated or not. I hope to 

 take up this question iater on. 



3. PlNUS Thunbergii. 

 In the decomposition products of the proteids and in the 

 etiolated shoots, arginin was isolated just in the same way as in 

 Cryptomeria japonica and was found to amount also to more 

 than half of the entire bases. 



IV. Organic bases in the decomposition products of the 

 proteids prepared from the shoots of 

 Pinus Thunbergii. 



It will be very interesting to see whether the chemical 

 nature of the proteids in the shoots of coniferous plants is differ- 

 ent from that of the proteids in seeds or not. For this purpose, 

 the shoots of Pinus Thunbergii io-i2cm. high grown in the full 

 day-light in the purified sea sand were dried and powdered and 

 its composition was determined first : — 



Average dry weight of IOOO shoots. 6.54 gram. 



In 100 parts of dry matter. 



Total nitrogen. 7.74 

 Albuminoid nitrogen. 3.40 

 Asparagine nitrogen. 1.42 

 Nitrogen in phospho-tungstic precipitate. 0 - 1 0.34 

 Other nitrogen. 2.58 



About 50 grams of the finely powdered sample were mixed 

 with about ioooc.c. of 0.2-0.2,% caustic soda solution, well stirred 

 and let stand for 24 hours, then filtered with clean cloth, and 



(i) By long exposure to day-light, organic bases had been reduced to the minimum 

 quantity. 



