ON THE FORMATION OF ARGININ IN CONIFEROUS PLANTS. 27 



2) 2% ammonium chloride solution, half saturated with 

 gypsum.* 



After twelve days (on the 27th), they were removed from 

 the sand, washed very well, until entirely free of ammonia, 

 dried and analyzed. 



As the temperature was too high, and the ammonium salt 

 too concentrated, some of the shoots treated with the ammonium 

 salt began to show signs of suffering. These affected shoots 

 were rejected and only the healthy ones were analyzed. 



a) b) c) • 



Shoots treated with 



Shoot treated 2% ammonium 



Shoots dried with J sat. chloride, half satu- 



on the gypsum for rated with gypsum 



15th March. 12 days for 12 days 



(I5th-27th). (i5th-27th). 



Number of shoots. 710 1656 r 340 



Length 6-8cm. 10-15 10-15 



Total dry weight. 4-250g. 15.051 12.353 



Dry weight of 100 shoots. o.59gg. 0.909 0.922 



In 100 parts of dry matter. 



a) b) c) 



Total nitrogen. 8.73 6.90 8.45 



Albuminoid nitrogen. 3.42 3.18 3.35 



Asparagine nitrogen. 1.24 1.15 1.20 

 Nitrogen in phospho- 



tungstic precipitate. 1.81 0.38 1.55 



Other nitrogen 2.26 2.19 2.35 



In 100 parts of total nitrogen. 



a) b) c) 



Total nitrogen. 100.0 100.0 100.0 



Albuminoid nitrogen. 39.2 46.1 39.6 



* A 2% ammonium chloride solution is evidently too strong for the young shoots, 

 but I prefered it, because a more dilute solution is too favorable to the growth of the 

 shoots, and the absorbed ammonium salts may be converted into proteids too rapidly, the 

 consequence being that no intermediate product can be detected. I had failed twice in 

 this way ; so it is necessary to give a concentrated solution, and to make the shoots 

 sutler a little, so that the regeneration of proteids may be retarded. Otherwise it is too 

 difficult to find out the intermediate product, stored up as such in the plant cells. 



