2S 



U. SUZUKI. 



-Tlo|Jcll tlL^lHL UlllUgCII. 



14.- 



10.7 



14.2 



Nitrogen in phospho- 









tungstic precipitate. 



20.7 



5.5 



10. 0 



Other nitrogen. 



25.9 



31-7 



27.9 



Every 100 shoots contain : — 







a) 



b) 



c) 



Total nitrogen. 



0.0523 



0.0627 



O.O779 



Albuminoid nitrogen. 



0.0210 



0.0289 



O.O3O9 



Asparagine nitrogen. 



0.0074 



0.0105 



O.OI I I 



Nitrogen in phospho- 









tungstic precipitate. 



0.0109 



0.0035 



0.0143 



Other nitrogen. 



0.0135 



0.0199 



0.0217 



The shoots dried on the 15th March a) were not fully 

 developed, and the reserve materials in the seeds were not yet 

 completely transported to the growing shoots. Therefore the 

 absolute quantity of every 100 shoots is considerably lower in 

 a), but after 12 days, that is in b) and c), almost all reserve ma- 

 terial was transported to the shoots. The considerable increase 

 of the absolute quantity of nitrogen in c) compared with b) may 

 be due to the absorption of the ammonium salt offered to the 

 shoots. Anyhow, we see from the above tables that even when 

 exposed to full day-light much organic bases* (that is, nitrogen 

 precipitated by phospho-tungstic acid) are accumulated in the 

 first stage of germination a), while they disappear very rapidly 

 on further exposure to day-light, the disappearance being most 

 probably due to their being directly used for the regeneration 

 of proteids, as we see in the shoots b). But we observe that 

 the shoots treated with ammonium chloride solution still contain 

 a considerable quantity of organic bases c). The greater part 

 of these organic bases must be synthetically formed from the 

 ammonium salt absorbed by the shoots. We shall see the rela- 

 tion more clearly if we calculate the total nitrogen of a) as 100. 

 Thus we have : — 



* About 20^ u ' of the nitrogen must have been transported from the seeds to the 

 shoots during the experiments (15th to 27th), and we see that in c) about 29% of the 

 nitrogen had been absorbed from the ammonium salts offered. 



