ON THE FORMATION OF ARGININ IN CONIFEROUS PLANTS. 31 



I have also tried to isolate arginin from the shoots treated 

 with gypsum solution only (b), but I found only a very small 

 quantity of arginin, while in the shoots dried on the 15th 

 March (a), there was a tolerably large quantity. In both cases, 

 however, it was found to be less than in the shoots treated with 

 ammonium salts (c). 



2. Second experiment with the shoots of 

 Pinus Thunbergii. 



Young shoots, grown in the purified sea sand, were carefully 

 removed from the sand ; one portion was directly dried, and the 

 other portion was divided into 2 parts and put in the following 

 solutions : — 



1) Half saturated gypsum solution. 



2 ) °-S% ammonium choride solution, half saturated with 

 gypsum. 



This second portion was kept in the laboratory in diffuse 

 day-light, at the temperature ranging between 10 0 C and 16 0 C. 

 After 12 days (from 27th March to 8th April), the shoots were 

 removed from the solutions, washed very well, until no trace 

 of ammonia was left, dried and analyzed. During the experi- 

 ment, the solutions were renewed four times to prevent bacterial 

 turbidity to appear. But, as toward the end of the experiment, 

 the shoots put in the ammonium chloride solution began to 

 suffer, while those kept in the gypsum solution were still 

 healthy, I could not keep them any longer. 



The analysis gave the following results : — 



a) 



b) 



Shoots 

 dried on 

 the 

 27th 

 March. 



Shoots kept in 

 gypsum solution 



for 12 days 

 (27th March- 

 8th April). 



ammonium chloride 

 solution for 12 days 



(27th March- 

 8th April). 



Number of shoots. 

 Length. 



Total dry weight. 

 Dry weight of every 



1 2-1 5cm. 

 I5-Q5ig- 



165G 



400 

 12-15 



3-955 



446 

 12-15 



4.296 



100 shoots. 



o.909g. 



0.989 



0.963 



