36 



U. SUZUKI. 



They were kept in the laboratory in diffuse day-light, the 

 temperature varying between io°C. and i5°C. After 7 days, 

 they were .removed from the solutions, Avashed well, dried and 

 analyzed. The solutions were renewed twice and no bacterial 

 growth was observed. Toward the end of the experiment, some 

 of the leaves began to suffer, and the top became yellow. These 

 weaklings were removed. The analysis gave the following 

 results : — 



In 100 parts of dry matter : — 





a) 



b)* 



c)* 





Plants, dried 



Plants kept in 



Plants kept in am- 





on 



gypsum solution 



monium sulphate 





9th Nov. 



(7-i 4 th). 



solution (7-i4th). 



Total nitrogen. 



4.90 



6.12 



8-35 



Albuminoid nitrogen. 



4.21 



2.88 



3-68 



Asparagine nitrogen. 



0.18 



1.48 



2.52 



Nitrogen in phospho- 









tungstic inrecipitate. 



0.28 



0.30 



0.36 



Other nitrogen. 



c.23 



1.46 



1.79 



Here we found no increase of organic bases ; on 



the contrary, 



much increase of asparagine was observed. 



2. HORDEUM D1STICHON. 



On the 7th November, young barley plants 10 cm. high, 

 were carefully removed from the field and washed well. A 

 portion was directly dried, while the other portion was divided 

 into tw o lots and grown in the following solutions : — 



1) Half saturated gypsum solution. 



2) 0.5% ammonium sulphate solution, half saturated with 

 gypsum. 



* During the experiment, much decomposition of proteids took place, in consequence 

 of which much increase of asparagine was observed in the control plants b). The 

 increase was, however, much greater in c), which must be due partly to synthetical 

 formation from the ammonium salt. 



The higher percentage of total nitrogen in b) was due to the decrease of dry matter 

 during the experiment and in c) and partly to the absorption of ammonium salt by the 

 roots. 



Here we need not calculate the absolute quantity of 100 shoots etc., the result being 

 too decisive. 



