ON THE FORMATION OF ARGININ IN CONIFEROUS PLANTS. 37 



They were kept in the laboratory in diffuse day-light, the 

 temperature ranging between io° C. and 15° C. After 7 days 

 they- were taken out from the solutions, washed, dried and 

 analyzed. 



In IOO parts of dry matter : — 





a) 



b)* 



c)* 







Plants, kept in 



Plants, kept in 





Plants, dried 



gypsum solution 



ammonium sul- 





on the 7th. 



for 7 days 



phate solution for 





( 7 th-i 4 th). 



7 days (7th-i4th). 



Total nitrogen. 



5.4O 



5.56 



6.60 



Albuminoid nitrogen. 



4.52 



3-04 



4.27 



Asparagine nitrogen. 



O.3O 



1.52 



I.42 



Nitrogen in phospho- 









tungstic precipitate. 



0.42 



0.52 



0.64 



Other nitrogen. 



0.16 



0.48 



0.27 



We found again no increase of organic bases in this case. 



3. Second experiment with the shoots 

 of Barley. 



(Hordeum distichon.) 



On the 2nd November, grains of barley were sown in four 

 wooden boxes containing purified saw dust. Two were kept in 

 the dark and the other two in full day-light in a warm house. 

 Germination had fairly set in and on the 14th the shoots w r ere 

 6-8 cm. high. One of the boxes of either set was then treated 

 with a half saturated gypsum solution and the other with a 

 O-5/o ammonium sulphate solution half saturated with gypsum. 

 After 7 days (on the 21st) the shoots were carefully removed 

 from the saw dust, washed well, dried and analyzed. No 

 ammonia was stored up in the shoots. The analysis gave the 

 following results : — 



In 100 parts of dry matter. 

 Plants kept in : — 



* During the experiment much decomposition of proteids took place, in consequence 

 of which much asparagine was formed. But we found no difference in the amount of 

 asparagine in b) and c) ; the asparagine once formed being perhaps transformed into 

 proteids. 



