3S U. SUZUKI. 



Dark. Light. 





a) 



b) 



a) 



b) 





Treated 



Treated with 



Treated 



Treated with 





with 



ammonium 



with 



ammonium 





gypsum. 



sulphate. 



gypsum. 



sulphate. 



Total nitrogen. 



3.82 



4.58 



3-7 2 



4.4O 



Albuminoid nitrogen. 



2.48 



2.56 



2.2 I 



2.4O 



Asparagine nitrogen. 



o.?o 



0.90 



0.94 



1.52 



Nitrogen in phospho- 











tungstic precipitate. 



0.26 



0.36 



0.24 



0.27 



Other Nitrogen. 



0.38 



0.76 



0-33 



0.21 



We see that the shoots treated with the ammonium sulphate 

 solution, produced both in the dark and in day light, much 

 asparagine, but no organic bases. 



4. CUCURBITA MELOPEPO (pumpkin). 



Un the 2nd November, pumpkin seeds were sown in purified 

 sea sand ; one portion was kept in the dark and the other 

 portion in day light in a warm house, where the temperature 

 ranged between 15 0 C. and 35° C. On the 16th, the shoots kept 

 in the dark were 5-13 cm. high and those kept in day light 4-8.5 

 cm. high ; then one-half of the shoots in both plots was removed 

 from the sand, washed and analysed, and the remaining half was 

 treated with a 0.5^ ammonium sulphate solution, half saturated 

 with gypsum. After 7 days (22nd) the shoots in the dark were 

 18 cm. high, while those in day light were 12 cm. high. Both 

 were then removed from the sand, washed well, dried and 

 analyzed. 



In 100 parts of dry matter. 

 Shoots kept in : — 



Total nitrogen. 

 Albuminoid nitrogen. 

 Asparagine nitrogen. 



Da 



rk. 



Lig 



ht. 



Control 



Treated with 





Treated with 



(dried on 



ammonium 



Control. 



ammonium 



the 16th). 



sulphate 





sulphate 





(16-22). 





(16-22). 



6.74 



8.65 



6.96 



8.62 



5-85 



5.59 



5.72 



4.87 



0.28 



1.36 



0.38 



2.09 



