ON THE FORMATION OF ARGININ IN CONIFEROUS PL A NTS. 41 

 Calculating the total nitrogen of a) as 100 we have : — 



a) b) 



Total nitrogen. 100.0 108.0 



Albuminoid nitrogen. 34. 1 33.3 



Asparagine nitrogen. 25.2 26.6 

 Nitrogen in phospho- 



tungstic precipitate. 17.5 21.3 



Other nitrogen. 23.2 26.3 



In the 2 weeks shoots, the reserve material in the seed has 

 not yet been completely transported to the shoots and a portion 

 still remained undissolved. Therefore we find a little increase 

 of total nitrogen in the 4 weeks shoots. We see from the above 

 results that a considerable amount of organic bases is already 

 formed in the first 2 weeks and after-wards increases only very 

 gradually. 



Plate III. shows the change of various nitrogenous 

 compounds (especially nitrogen in phospho-tungstic precipitate) 

 during the developement of the shoots of Pinus Thunbergii 

 in perfect darkness. 



2. The shoots were kept in full day-light and harvested in 

 4 different periods to see the change in nitrogen compounds. 





a) 



b) 



c) 



d) 



Shoots dried after 



14 



19 



28 



32 days 



( 9 th 



March) (14th) 



(23rd) 



(27th) 



Number of shoots. 



1348 



7IO 



780 



1656 



Length. 



6-8 



8-10 



10-15 



12-16 



Total dry weight. 



8.672 



4.750 



7.185 



15-533 



Dry weight of 100 shoots. 



0.643 



O.669 



0.921 



0.938 



In 100 parts of dry matter. 









a) 



b) 



c) 



d) 



Total nitrogen. 



8.81 



3-73 



6.95 



6.90 



Albuminoid nitrogen. 



3-5i 



3-8i 



3-27 



3.29 



Asparagine nitrogen. 



1.32 



1.24 



1.26 



1. 15 



Nitrogen in phospho- 











tungstic precipitate. 



1.54 



1.62 



0.44 



0.38 



Other nitrogen. 



2.44 



2.06 



1.98 



2.08 



