S 4 



K. A So. 



following way : 16.69 grams of the dry spores (1) were extracted 

 with dilute caustic soda (2) after extracting with ether and alcohol, 

 and some dilute hydrochloric acid was added until the solution 

 gave a faintly acid reaction. 



After filtering and well washing with dilute hydrochloric 

 acid (about 0.2%), the dark brown precipitate was mixed with 

 250 c.c. of gastric juice and digested in the usual way in a water 

 bath for eighteen hours at 40°C. ; then filtered and thoroughly 

 washed with dilute hydrochloric acid. The residue was dis- 

 solved in dilute ammonia, and again precipitated by making the 

 ammoniacal solution faintly acid with dilute hydrochloric acid ; 

 then filtered. The precipitate was collected on a weighed filter 

 and thoroughly washing first with dilute hydrochloric acid and 

 then with water, washed with boiling alcohol and ether. Here- 

 upon I obtained a brown mass which was weighed after drying 

 at ioo c C. 16.69 grams, of the dry spores yielded 0.59 grams of the 

 brown mass, that is, 3.535,% in the dry spore. This brown mass 

 contained lo.&% nitrogen. 



It seemed to me of interest to determine the presence of 

 some bases, if any, in the spores. About 10 grams of the dry 

 spores <3) were extracted with boiling water, and from this aque- 

 ous solution a precipitate was obtained with basic lead acetate. 

 After filtering, the excess of lead in the filtrate was precipitated 

 by sulphuretted hydrogen, and filtered. After evaporating to a 

 small volume, adding a little sulphuric acid, phospho-tungstic 

 acid was added to the filtrate, whereby the characteristic pre- 

 cipitate was obtained. The phospho-tungstic precipitate was 

 first washed with cold water containing some sulphuric acid, and 

 then decomposed with caustic baryta, and the filtrate evaporated 

 after removal of the excess of baryta by means of carbonic acid. 

 The evaporated solution (4) was treated with an ammoniacal 

 solution of silver nitrate, whereby a white precipitate was 

 obtained. The precipitate thus obtained was collected on a 



(1) The third sample. 



(2) The spores could not be digested completely unless I treated them first with 

 alkalies, because some of them floated on the surface of the gastric juice and could not 

 be brought into intimate contact with the juice. 



(3) The third sample. 



(4) I applied the biuret test for peptone, but no reaction was obtained. 



