THE CHEMICAL COMPOSITION OF THE SPORES. 



8 9 



boiling with dilute hydrochloric acid. It produced oxalic acid 

 upon boiling with strong nitric acid. There can hardly be any 

 doubt that this substance was trehalose (mycose). The quanti- 

 ty of dextrose obtained after boiling the alcoholic extract might 

 be equivalent to that of glucose, which was split from 

 trehalose. 



III. Mineral Constituents of the Spores of 

 Aspergillus Oryzae. 



1. Total Ash. 



The ash of the spores was estimated after incineration and 

 determination of the carbon and carbonic acid contained therein, 

 which were deducted from the raw ash. The result is shown in 

 the following table : 





First sample. 



Second sample. 



Third sample. 



Total ash in dry 











4.844% 



6.112% 



matter. 









2. Mineral Constituents. 



The ash-analysis (0 was carried out by the usual method for 

 vegetable ash with the following result : 



In 100 parts of the ash, 



K 2 0 45.964 



Na 2 0 4.131 



CaO 1.038 



MgO 4.364 



Fe 2 0 3 4.916 



P 2 0 5 39.640 



S0 3 2.000 



Si0 2 0.409 



The test for chlorine was made and its presence in the ash 

 distinctly proved. 



(1) The ash of the second sample was here used. 



