U. SUZUKI. 



26l 





Nuclcin. 



Bunge's 

 Haematogen 



f r*r\rri cct ct v ri\ 1." 

 1 l ' '111 t L, yuiJ\. 



Haemoglobin. 



Stoklasa's 

 Haematogen 



f mm T"»l 1 11 f *i 

 1 1 1 1 1 1 1 1 <i ii 1 1 1 0. 



c 



4O.81 



42. 1 1 



54.26 



4305 



H 



5.38 



6.08 



7.IO 



5-56 



IN 



15.98 



'4-73 



l6.2 I 





s 



O.38 



o.55 



O.54 



0.28 



p 



6. 19 



5.19 



°77( p 2° 



5) 6.21 



Fe 





0.29 



043 



1.68 



0 



31.26 



31-05 



20.69 



28.08 



We see how closely nuclein and haematogen agree in 

 composition. 1 Both chemical and microscopical examination of 

 seeds by Bungc has shown that the iron is localized in the 

 embryo and endosperm only in an organic combination. Most 

 probably the haematogen is used for the formation of the 

 cellular nucleus. Stoklasa tried in vain to isolate haematogen 

 from young maize plants cultivated in a solution containing no 

 iron while those cultivated in a solution containing iron, gave 

 a moderate quantity of it. Lower plants containing no chloro- 

 phyll require nevertheless, according to Molisch, iron just as 

 chlorophyll bearing plants do. Thus, Mucor mucedo or Bacillus 

 megatherium never develop well in a solution free from iron. 

 Certain fungi contain also haematogen ; thus from iooog. of 

 Boletus edulis 3-5g- haematogen was isolated by Stoklasa, and he 

 therefore suggested that iron is an essential constituent of 

 nuclcin like phosphoric acid. K.Aso 3 has shown that the 

 spores of Aspergillus oryzae contain relative!}' much iron (about 



Fe 2 O s in the ash). On treating the spores with dilute 

 alkali and neutralizing the alkaline extract with acetic acid, a 

 protein substance was obtained, which, after being subjected to 

 artificial pepsin digestion, yielded an insoluble residue containing 

 iron. This compound was again dissolved in ammonia, the 

 ammoniacal solution again neutralized with hydrochloric acid, 

 and even after this purification process the nuclein obtained was 

 tolerably rich in iron. It is especially interesting that this 



1 Recently Hius"rmann found a small quantity of iron in the serum of blood, (1 

 nig. in 100 c.c.). Dried fibrin from the dogs blood contained 0.01% iron. 



2 Bui, Cell, of Agriculture Vol. Ill No. 1. 



